Bioremediation of Cadmium, Lead, Zinc and Copper Contaminated Soils obtained from Dutse and Hadejia Regions by Lysinibacillus sphaericus Strain FUD-001 and Stenotrophomonas maltophilia Strain FUD-002 Immobilized on a Biochar
DOI:
https://doi.org/10.56919/usci.2541.002Keywords:
Immobilization, Bioremediation, Biochar, Heavy-Metals, Atomic Absorption SpectroscopyAbstract
Toxic heavy metals have a detrimental impact on the human body and trigger acute or chronic effects or lead to cancer and death, gastrointestinal tract disorder, and nervous system breakdown. An efficient approach for the amelioration and restoration of soil heavy metal contamination is through bioremediation using biochar-immobilized bacteria. Soil samples were analyzed for Cadmium, Lead, Zinc, and Copper using Atomic Absorption Spectroscopy (AAS), and the isolates were subjected to heavy metal tolerant tests for Cd, Pb, Zn, and Cu at 50mg/L, 80mg/L, 100mg/L, and 150mg/L. The isolates were immobilized on a biochar produced from orange peels (Citrus sinensis) after morphological, microscopic, biochemical, and molecular identification, then subjected to a test for bioremediation potential for Cd, Pb, Zn, and Cu at concentrations of 10mg/L, 25mg/L, and 50mg/L. Biochar-immobilized Lysinibacillus sphaericus Cd percentage removal at 10mg/L, 25mg/L, and 50mg/L was 99.3, 99.7, and 99.8, respectively. Pb percentage removal was 100 for all three concentrations. Cd percentage removal for biochar-immobilized Stenotrophomonas maltophilia at 10mg/L, 25mg/L, and 50mg/L was 99.3, 99.7, and 99.8, respectively. Pb percentage removal at the same concentrations was 100. Orange peel was found to be a suitable substrate for biochar production and immobilization of bacteria. Chemical activation of the biochar using HNO3 improved its sorption capacity. Based on the result obtained, the biochar-bacteria complex is a highly remarkable strategy and can be employed in the bioremediation of heavy metal-contaminated soils.
References
Adeniyi, A., G., Joshua, O., I., Damilola, V., Onifade. (2020). Biochar from the Thermochemical Conversion of Orange (Citrus sinensis) Peel and Albedo: Product Quality and Potential Applications. Chemistry Africa. https://doi.org/10.1007/s42250-020-00119-6
Agarwal, D., Dhevang, R., Shreyas, T., Prachi, B., Sejal, R. (2021). Isolation of Pseudomonas species from soil sample for production of Pyoverdine and evaluation of its potential as an antimicrobial agent. International Journal of Scientific Research in Science and Technology Print ISSN: 2395-6011 | Online ISSN: 2395-602X (www.ijsrst.com) doi: https://doi.org/10.32628/IJSRST218555
Agnello, A., C., Bagard, M., van Hullebusch, E., D., Esposito, G., Huguenot, D. (2016) Comparative bioremediation of heavy metals and petroleum hydrocarbons co-contaminated soil by natural attenuation, phytoremediation, bioaugmentation and bioaugmentation-assisted phytoremediation. SciTotal Environ 563:693-703. https://doi.org/10.1016/j.scitotenv.2015.10.061
Ahsan, N., and Shimizu, M. (2021). Lysinibacillus Species: Their Potential as Effective Bioremediation, Biostimulant, and Biocontrol Agents. Reviews in Agricultural Science, 9:103–116, https://dx.doi.org/10.7831/ras.9.0_103
Carver, A., Gallicchio, V., S. (2018) Cancer Causing Substances. IntechOpen; London. Chapter 1: Heavy metals and cancer. https://doi.org/10.5772/intechopen.70348
Chen, T., Jing, W., Feng, G., Ying, L., Yuhuan, Sun., Yongming, L. (2021). Biochar and bacteria inoculated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soil. Adv. Environ. Technol. 2017, 2, 179–184.
Chen, Y., Y., Wu, H., X., Sun, P., Liu, J., X., Qiao, S., X., Zhang, D., K., Zhang, Z., M. (2022). Remediation of chromium-contaminated soil based on Bacillus cereus WHX-1 immobilized on biochar: Cr(VI) transformation and functional microbial enrichment. Front Microbiol, 12:10. https://doi.org/10.3389/fmicb.2021.641913
Chen, H., Zhang, J., Tang, L., Su, M., Tian, D., Zhang, L., Li, Z., Hu, S., 2019. Enhanced Pb immobilization via the combination of biochar and phosphate solubilizing bacteria. Environ. Int. 127:395–401. https://doi.org/10.1016/j.envint.2019.03.068
Chen, S., Yin, H., Ye, J., Peng, H., Liu, Z., Dang, Z., and Chang, J. (2014). Influence of coexisted benzoapyrene and copper on the cellular characteristics of Stenotrophomonas maltophilia during biodegradation and transformation. Bioresour Technol, 158: 181–187. https://doi.org/10.1016/j.biortech.2014.02.020
Galitskaya, P., G., Leisan, A., and Svetlana, S. (2016). Biochar-carrying hydrocarbon decomposers promote degradation during the early stage of bioremediation. Biogeosciences, 13, 5739–5752, 2016. https://doi.org/10.5194/bg-13-5739-2016
Garza-Lombo, C., Posadas, Y., Quintanar, L., Gonsebatt, M., E., Franco, R. (2018). Neurotoxicity linked to dysfunctional metal ion homeostasis and xenobiotic metal exposure: Redox Signaling and Oxidative Stress. Antioxid. Redox Signal. 28:1669–1703. https://doi.org/10.1089/ars.2017.7272
Gerhardsson, L., Kazantzis, G. (2015). Handbook on the Toxicology of Metals. 4th ed. Academic Press; Cambridge, MA, USA: 2015. Chapter 23: Diagnosis and treatment of metal poisoning: General aspects; pp. 487–505. https://doi.org/10.1016/B978-0-444-59453-2.00023-8
Guo, H., Liu, H., Wu, H., Cui, H., Fang, J., Zuo, Z., Deng, J., Li, Y., Wang, X., Zhao, L. (2019). Nickel carcinogenesis mechanism: DNA damage. Int. J. Mol. Sci. 20:4690. https://doi.org/10.3390/ijms20194690
Kang, C., H., Han S., H., Shin, Y., J., Oh, S., J., So, J., S. (2014). Bioremediation of Cd by microbially induced calcite precipitation. ApplBiochemBiotechnol 172:1929-193. https://doi.org/10.1007/s12010-013-0626-z
Kang, C., H., Oh, S., J., Shin, Y., J.0, Han, S., H., Nam, I., H., et al. (2015). Bioremediation of lead by ureolytic bacteria isolated from soil at abandoned metal mines in south korea. EcolEng 74:402-407. https://doi.org/10.1016/j.ecoleng.2014.10.009
Kang, C., H., So, J., S. (2016). Heavy metal and antibiotic resistance of ureolytic bacteria and their immobilization of heavy metals. EcolEng 97:304-312. https://doi.org/10.1016/j.ecoleng.2016.10.016
Kim, H., S., Kim, Y., J., Seo, Y., R. (2015). An overview of carcinogenic heavy metal: Molecular toxicity mechanism and prevention. J. Cancer Prev. 20:232–240. https://doi.org/10.15430/JCP.2015.20.4.232
Kim, H., S., Kim, Y., J., Seo, Y., R. (2015). An overview of carcinogenic heavy metal: Molecular toxicity mechanism and prevention. J. Cancer Prev. 20:232–240. https://doi.org/10.15430/JCP.2015.20.4.232
Li., L., Shoutong, W., Xiangzhen, L., Tiezhu, Li., Xiaohong, H., Yong, T. (2018). Effects of Pseudomonas chenduensis and biochar on cadmium availability and microbial community in the paddy soil. Science of the Total Environment, 640-641:1034-1043. https://doi.org/10.1016/j.scitotenv.2018.05.287
Ma, H., Mingyang, W., Ziru, W., Siyu, H., Xuedan, L., Heng, X. (2020). Bioremediation of cadmium polluted soil using a novel cadmium immobilizing plant growth promotion strain Bacillus sp. TZ5 loaded on a biochar. Journal of Harzadous Materials, 388:122065. https://doi.org/10.1016/j.jhazmat.2020.122065
Mohammed, S., E., (2021). Spatial Assessment of Some Heavy Metals in Hadejia Nguru Wetlands, Nigeria. International Journal of Water Resources and Arid Environments ISSN 2079-7079, 10(1): 20-24.
Naghipour, D., Seyed, D., A., Mozhgan, G., Kamran, T., Mohammad, N-J. (2018). Phytoremediation of heavy metals (Ni, Cd, Pb) by Azolla filiculoides from aqueous solution: A dataset. Data in Brief. 1409–1414. https://doi.org/10.1016/j.dib.2018.10.111
Nath, S., Payal, P., Rajdeep, R., Satabdi, B., Bibhas, D. (2019). Isolation and identification of metal tolerant and antibiotic resistant bacteria from soil samples of Cachar district of Assam, India. https://doi.org/10.1007/s42452-019-0762-3
Ogunnusi, T., A., and Oyetunji, O., A. (2017). Isolation of indigenous microorganisms from soil contaminated with metal scraps for the uptake of selected heavy metals in constituted growth media. Vol. 11(46), pp. 1643-1648, DOI: 10.5897/AJMR2017.8594 Article Number: 5E5F47C67003 ISSN 1996-0808. https://doi.org/10.5897/AJMR2017.8594
Peng, P., Lang, Y., H., Wang, X., M. (2016). Adsorption behavior and mechanism of pentachlorophenol on reed biochars: pH effect, pyrolysis temperature, hydrochloric acid treatment and isotherms. Ecol Eng., 90:225–233. https://doi.org/10.1016/j.ecoleng.2016.01.039
Pietrzak, S., Wójcik, J., Baszuk, P., Marciniak, W., Wojtyś, M., Dębniak, T., Cybulski, C., Gronwald, J., Alchimowicz, J., Masojć, B., et al. (2021). Influence of the levels of arsenic, cadmium, mercury and lead on overall survival in lung cancer. Biomolecules. 11:1160. https://doi.org/10.3390/biom11081160
Qi, X., Jialei, G., Xiaoming, C., Shiqi, X., Imran, A., Ran, S., Dan, W., Yuewen, W., Mengwei, H., Xuegang, L. (2021). Application of mixed bacteria-loaded biochar to enhance uranium and cadmium immobilization in a co-contaminated soil, Journal of Hazardous Materials, 401:123823. https://doi.org/10.1016/j.jhazmat.2020.123823
Qiu, M., Liu, L., Ling, Q., Cai, Y., Yu, S., Wang, S., Fu, D., Hu, B., Wang, X. (2022) Biochar for the removal of contaminants from soil and water: a review. Biochar 4:19. https://doi.org/10.1007/s42773-022-00146-1
R, Amen. (2020). A critical review on arsenic removal from water using biochar-based sorbents: the significance of modification and redox reactions Chem. Eng. J. https://doi.org/10.1016/j.cej.2020.125195
Romaniuk, A., Lyndin, M., Sikora, V., Lyndina, Y., Romaniuk, S., Sikora, K. (2017). Heavy metals effect on breast cancer progression. J. Occup. Med. Toxicol. 12:32. https://doi.org/10.1186/s12995-017-0178-1
Sani, A., Khadija, M., I., Bukar, A., A., Aminu, I., D. (2022). Bioaccumulation and health risks of some heavy metals in Oreochromis niloticus, sediment and water of Challawa river, Kano, Northwestern Nigeria. Environmental Advances. (2022) 100172. https://doi.org/10.1016/j.envadv.2022.100172
Tu, C., Wei, J., Guan, F., Liu, Y., Sun, Y., Luo, Y., 2020. Biochar and bacteria inoculated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soil. Environ. Int. 13:105576. https://doi.org/10.1016/j.envint.2020.105576
Wang, L., Zhangtao, Li, Y., W., Philip, C., B., Fan, W., Qichun, Z., Jianming, X., Xingmei, L. (2021). Performance and mechanisms for remediation of Cd(II) and As(III) co-contamination by magnetic biochar-microbe biochemical composite: Competition and synergy effects. https://doi.org/10.1016/j.scitotenv.2020.141672
Witkowska, D., Joanna, S., and Karolina, C. (2021). Heavy Metals and Human Health: Possible Exposure Pathways and the Competition for Protein Binding Sites. Molecules. 26(19): 6060. https://doi.org/10.3390/molecules26196060
Wu, B., Wang, Z., Zhao, Y., Gu, Y., Wang, Y., Yu, J., Xu, H., (2019). The performance of biochar-microbe multiple biochemical material on bioremediation and soil microecology in the cadmium aged soil. Sci. Total Environ. 686, 719–728. https://doi.org/10.1016/j.scitotenv.2019.06.041
Wu, J., W., Wang, T., Wang, J., W., Zhang, Y., S., Pan, W., P. (2020). A novel modified method for the efficient removal of pb and cd from wastewater by biochar: enhanced the ion exchange and precipitation capacity. Sci Total Environ 754:10. https://doi.org/10.1016/j.scitotenv.2020.142150
Yang, X., Zhang, S., Ju, M., Liu, L. (2019). Preparation and modification of biochar materials and their application in soil remediation. Appl Sci, 9:1365. https://doi.org/10.3390/app9071365
Zhou, N., Chen, H., Feng, Q., Yao, D., Chen, H., Wang, H., Zhou, Z., Li, H., Tian, Y., Lu, X. (2017). Effect of phosphoric acid on the surface properties and Pb (II) adsorption mechanisms of hydrochars prepared from fresh banana peels. J Clean Prod, 165:221–230. https://doi.org/10.1016/j.jclepro.2017.07.111
Zhou, X., Wang, R., Liu, H., Wu, S., Wu, H. (2019) Nitrogen removal responses to biochar addition in intermittent- 679 aerated subsurface flow constructed wetland microcosms: Enhancing role and mechanism. Ecological Engineering. 128: 57-65. https://doi.org/10.1016/j.ecoleng.2018.12.028
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