Development of Sterilization protocol for In Vitro Germination of Prosopis Africana on Cocopeat as a low-Cost Alternative
DOI:
https://doi.org/10.56919/usci.2543.016Abstract
Prosopis africana, a valuable tree species in West Africa, is the only species of Prosopis that is indigenous to tropical Africa. Due to extensive overexploitation, this species has become extinct in many parts of the southern Sahel and the adjacent Sudan savannahs. In the first experiment, scarified seeds were surface sterilized with 10% and 20% NaOCl solution for 10 and 20 minutes. In the second experiment, seeds were soaked in 1% antifungal solution for 30 and 60 minutes before final sterilization with 10% and 20% NaOCl. Treating seeds with 10% and 20% NaOCl alone for 10 and 20 minutes resulted in very low contamination levels of 5.0 ± 0.04 and 2.5 ± 0.03, respectively, with no significant difference between the treatments at p < 0.05. Seed germination was influenced by treatments, with significant differences at p < 0.05, where the highest germination was obtained with 20% NaOCL for 10 minutes (70.0 ± 13.00). In the second experiment, the lowest contamination (20% ± 2.41) and the highest germination (50% ± 14.1) were recorded for seeds treated with 10% NaOCL + 1% antifungal for 30 min. The best sterilization treatment was 20% NaOCL for 10 minutes, which had the highest germination and one of the lowest contamination percentages. Cocopeat can be successfully used as a low-cost medium to generate sterile explants of P. africana, which can be used for micropropagation and genetic improvement of the species. This can be used for the conservation of the threatened tree in Nigeria.
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