Phenotypic and Molecular Profiling of Multidrug-Resistant Staphylococcus aureus Isolated from Labour Rooms in Selected Hospitals of Katsina State, Nigeria
DOI:
https://doi.org/10.56919/10.56919/usci.2651.025Keywords:
Staphylococcus aureus, multidrug resistance, quinolone resistance, PCR, labour roomAbstract
Fluoroquinolone antibiotics are widely used in managing infections caused by multidrug-resistant (MDR) Staphylococcus aureus. However, the emergence and spread of resistance to these agents pose serious public health threats, particularly in healthcare environments across Katsina State, Nigeria. This study investigated the presence of selected quinolone resistance genes in MDR S. aureus isolates obtained from labour room environments in four hospitals within the state. A total of 240 environmental swab samples were collected and analyzed for the presence of S. aureus. Isolates were identified using standard biochemical methods, and their antibiotic susceptibility profiles were determined via the disk diffusion method. MDR status was defined as resistance to three or more antibiotic classes. Species confirmation was performed using PCR targeting the nuc gene, followed by detection of quinolone resistance genes (qnrA, qnrD, and parC) in the confirmed MDR isolates using PCR. Twenty-eight S. aureus isolates were recovered. High levels of resistance were observed to amoxicillin-clavulanate (100%), cefoxitin (100%), erythromycin (54.5%), and ciprofloxacin (54.5%). Fourteen isolates (50%) were identified as MDR. Among the five selected MDR isolates, PCR confirmed all as S. aureus, with 80% harbouring qnrD, 40% harbouring qnrA, and 20% harbouring parC. These findings highlight a significant burden of MDR S. aureus and associated resistance genes in labour room environments, underscoring the urgent need for strengthened infection prevention practices and responsible antibiotic use to mitigate further dissemination within maternity healthcare settings.
References
Ahmed, S. K., Hussein, S., Qurbani, K., Ibrahim, R. H., Fareeq, A., Mahmood, K. A., & Mohamed, M. G. (2024). Antimicrobial resistance: Impacts, challenges, and future prospects. Journal of Medicine, Surgery, and Public Health, 2, 100081. DOI: https://doi.org/10.1016/j.glmedi.2024.100081
Ajekiigbe, V. O., Ogieuhi, I. J., Odeniyi, T. A., Ogunleke, P. O., Olatunde, J. T., Babalola, A. V., & Anthony, C. S. (2025). Understanding Nigeria's antibiotic resistance crisis among neonates and its future implications. Discover Public Health, 22(1), 28. DOI: https://doi.org/10.1186/s12982-025-00422-y
Badamasi, A., & Salisu, B. (2025). Characterization of heavy metal-tolerant bacteria from dumpsites in Katsina Metropolis and their bioremediation potential. Umyu Scientifica, 4(2), 417–428. DOI: https://doi.org/10.56919/usci.2542.044
Chirca, I. (2019). The hospital environment and its microbial burden: Challenges and solutions. Future Microbiology, 14(12), 1007–1010. DOI: https://doi.org/10.2217/fmb-2019-0140
Clinical and Laboratory Standards Institute. (2022). Performance standards for antimicrobial susceptibility testing (32nd ed.; CLSI supplement M100).
de Oliveira, T. L. R., Cavalcante, F. S., Chamon, R. C., Ferreira, R. B. R., & Dos Santos, K. R. N. (2019). Genetic mutations in the quinolone resistance-determining region are related to changes in the epidemiological profile of methicillin-resistant Staphylococcus aureus isolates. Journal of Global Antimicrobial Resistance, 19, 236–240. DOI: https://doi.org/10.1016/j.jgar.2019.05.026
Ezeh, C. K., Eze, C. N., Dibua, M. E. U., & Emencheta, S. C. (2023). A meta-analysis on the prevalence of resistance of Staphylococcus aureus to different antibiotics in Nigeria. Antimicrobial Resistance & Infection Control, 12(1), 40. DOI: https://doi.org/10.1186/s13756-023-01243-x
Gall, E., Long, A., & Hall, K. K. (2020). Infections due to other multidrug-resistant organisms. In Making healthcare safer III: A critical analysis of existing and emerging patient safety practices. Agency for Healthcare Research and Quality.
Garvey, M. (2024). Neonatal infectious disease: A major contributor to infant mortality requiring advances in point-of-care diagnosis. Antibiotics, 13(9), 877. DOI: https://doi.org/10.3390/antibiotics13090877
Hor, S. Y., Hooker, C., Iedema, R., Wyer, M., Gilbert, G. L., Jorm, C., & O'Sullivan, M. V. N. (2017). Beyond hand hygiene: A qualitative study of the everyday work of preventing cross-contamination on hospital wards. BMJ Quality & Safety, 26(7), 552–558. DOI: https://doi.org/10.1136/bmjqs-2016-005878
Ioannou, P., Kofteridis, D. P., Alexakis, K., Koutserimpas, C., Papakitsou, I., Maraki, S., & Samonis, G. (2022). Candida species isolation from hospitalized patients with COVID-19—A retrospective study. Diagnostics, 12(12), 3065. DOI: https://doi.org/10.3390/diagnostics12123065
Iwalokun, B. A., Akinloye, O., Udoh, B. E., & Akinyemi, K. O. (2019). Efficacy of silver nanoparticles against multidrug resistant clinical Staphylococcus aureus isolates from Nigeria. Journal of Immunoassay and Immunochemistry, 40(2), 214–236. DOI: https://doi.org/10.1080/15321819.2018.1555765
Jauro, S., Hamman, M. M., Malgwi, K. D., Musa, J. A., Ngoshe, Y. B., Gulani, I. A., Kwoji, I. D., Iliya, I., Abubakar, M. B., & Fasina, F. O. (2022). Antimicrobial resistance pattern of methicillin-resistant Staphylococcus aureus isolated from sheep and humans in Veterinary Hospital Maiduguri, Nigeria. Veterinary World, 15(4), 1141–1148. DOI: https://doi.org/10.14202/vetworld.2022.1141-1148
Johnson, G. A. (2021). Antibiotic resistance profiles and population structure of healthcare-associated and community-associated Staphylococcus aureus isolated in Fort Portal, Western Uganda [Doctoral dissertation, University of Salford].
Jouhar, R. (2020). Diabetic foot ulcers: A review of the current concepts in pathogenesis, diagnosis, and management. Journal of Clinical Medicine Research, 12(3), 147–157.
Kadhum, D. A., Hamad, E. M., & Fahad, M. A. (2024). Molecular characteristics and antimicrobial resistance profiles of Staphylococcus aureus isolates from burns. Molecular Biology Reports, 51(1), 903. DOI: https://doi.org/10.1007/s11033-024-09837-3
Kareem, S. M., Al-Kadmy, I. M., Kazaal, S. S., Mohammed Ali, A. N., Aziz, S. N., Makharita, R. R., & Hetta, H. F. (2021). Detection of gyrA and parC mutations and prevalence of plasmid-mediated quinolone resistance genes in Klebsiella pneumoniae. Infection and Drug Resistance, 14, 555–563. DOI: https://doi.org/10.2147/IDR.S275852
Kumari, H., Chakraborti, T., Singh, M., Chakrawarti, M. K., & Mukhopadhyay, K. (2020). Prevalence and antibiogram of coagulase negative Staphylococci in bioaerosols from different indoors of a university in India. BMC Microbiology, 20(1), 211. DOI: https://doi.org/10.1186/s12866-020-01875-8
Long, D. R., Bryson-Cahn, C., Waalkes, A., Holmes, E. A., Penewit, K., Tavolaro, C., & Salipante, S. J. (2024). Contribution of the patient microbiome to surgical site infection and antibiotic prophylaxis failure in spine surgery. Science Translational Medicine, 16(742), eadk8222. DOI: https://doi.org/10.1126/scitranslmed.adk8222
Mahapatra, A., Patro, A. R. K., Khajuria, A., Dhal, S., & Praharaj, A. K. (2022). Ciprofloxacin-resistant Gram-negative isolates from a tertiary care hospital in Eastern India with novel gyrA and parC gene mutations. Medical Journal Armed Forces India, 78(1), 24–31. DOI: https://doi.org/10.1016/j.mjafi.2019.10.002
Medugu, N., Nwajiobi-Princewill, P. I., Shettima, S. A., Mohammed, M., Mohammed, Y., Wariso, K., Akujobi, C. N., Oduyebo, O. O., & Iregbu, K. C. (2021). A mini-national surveillance study of resistance profiles of Staphylococcus aureus isolated from clinical specimens across hospitals in Nigeria. Nigerian Journal of Clinical Practice, 24(2), 225–232. DOI: https://doi.org/10.4103/njcp.njcp_296_20
Melariri, H., Freercks, R., van der Merwe, E., Ten Ham-Baloyi, W., Oyedele, O., Murphy, R. A., & Melariri, P. E. (2024). The burden of hospital-acquired infections (HAI) in sub-Saharan Africa: A systematic review and meta-analysis. EClinicalMedicine, 71, 102571. DOI: https://doi.org/10.1016/j.eclinm.2024.102571
Merza, N. S., & Jubrael, M. S. J. (2015). Phylogenetic grouping of uro-pathogenic Escheiria [sic] coli using different molecular typing methods in Kurdistan Region-Iraq. International Journal of Chemical and Biomolecular Science, 1(4), 284–291. http://www.aiscience.org/journal/ijcbs
Mohamed, R. A. E., Moustafa, N. M., Gaber, S. A. B., & Elsaadawy, Y. S. (2024). Distribution of toxin-encoding genes among Staphylococcus aureus clinical isolates and their correlation with antibiotic resistance. Microbes and Infectious Diseases, 5(4), 1353–1366. DOI: https://doi.org/10.21608/mid.2024.287792.1943
Oche, D. A., Oyegoke, P. I., Akpudo, M. O., & Olayinka, B. O. (2021). Antibiotics resistance pattern of Staphylococcus aureus isolated from in-patients of an Orthopaedic Hospital in North-Western Nigeria. Epidemiology and Health System Journal, 8(4), 160–166. DOI: https://doi.org/10.34172/ijer.2021.29
Onyedibe, K. I., Shehu, N. Y., Pires, D., Isa, S. E., Okolo, M. O., Gomerep, S. S., & Pittet, D. (2020). Assessment of hand hygiene facilities and staff compliance in a large tertiary health care facility in northern Nigeria: A cross sectional study. Antimicrobial Resistance & Infection Control, 9(1), 30. DOI: https://doi.org/10.1186/s13756-020-0693-1
Protano, C., Cammalleri, V., Romano Spica, V., Valeriani, F., & Vitali, M. (2019). Hospital environment as a reservoir for cross transmission. Cleaning and disinfection procedures. Annali di Igiene Medicina Preventiva e di Comunita, 31(5), 436–448.
Richter, D. C., Heininger, A., Chiriac, U., Frey, O. R., Rau, H., Fuchs, T., & Weigand, M. A. (2022). Antibiotic stewardship and therapeutic drug monitoring of β-Lactam antibiotics: Is there a link? An opinion paper. Therapeutic Drug Monitoring, 44(1), 103–111. DOI: https://doi.org/10.1097/FTD.0000000000000949
Salehi, A., Rahimi, M. K., & Bagheri, F. (2025). The antibacterial efficacy and effect of tungsten nanoparticles (WO3) on the expression of tetracycline and erythromycin-resistance genes in Streptococcus agalactiae isolated from pregnant women. GMS Hygiene and Infection Control, 20, Doc40.
Salisu, B., Anua, M. S., Wan Ishak, W. R., & Mazlan, N. (2024). A polyphasic characterization of Aspergillus section Flavi isolated from Malaysian and Nigerian food grains and poultry feeds by phenotypic, chemotypic, and molecular methods. Biocatalysis and Agricultural Biotechnology, 58, 103217. DOI: https://doi.org/10.1016/j.bcab.2024.103217
Salisu, B., Ibrahim, F., Kaware, M. S., & Isah, M. (2025). Gas chromatographic evaluation of hydrocarbon degradation capabilities of Phyllosphere-derived Bacteria in simulated bioremediation of contaminated soil. UMYU Journal of Microbiology Research (UJMR), 10(1), 21–31. DOI: https://doi.org/10.47430/ujmr.25101.003
Shariati, A., Arshadi, M., Khosrojerdi, M. A., Abedinzadeh, M., Ganjalishahi, M., Maleki, A., & Khoshnood, S. (2022). The resistance mechanisms of bacteria against ciprofloxacin and new approaches for enhancing the efficacy of this antibiotic. Frontiers in Public Health, 10, 1025633. DOI: https://doi.org/10.3389/fpubh.2022.1025633
Sloan, S., Jenvey, C. J., Piedrafita, D., Preston, S., & Stear, M. J. (2021). Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes. BMC Biotechnology, 21(1), 35. DOI: https://doi.org/10.1186/s12896-021-00695-6
Thakur, H., & Rao, R. (2024). Emphasis of infection prevention and control: A review. Journal of Population Therapeutics and Clinical Pharmacology, 31, 2238–2249.
Tigabu, A., & Getaneh, A. (2021). Staphylococcus aureus, ESKAPE Bacteria challenging current health care and community settings: A literature review. Clinical Laboratory, 67(7). DOI: https://doi.org/10.7754/Clin.Lab.2020.200930
Uzoma, A., Kachi, U., & Martina, N. (2025). Prevalence of extended spectrum beta-lactamase producing bacteria in patients with wound infections attending tertiary hospitals in Enugu, Nigeria. South Asian Journal of Research in Microbiology, 19(2), 1–13. DOI: https://doi.org/10.9734/sajrm/2025/v19i2416
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