In-vitro Inhibition of Non-fluorescent - Non-crosslinking AGEs by Borassus aethiopum Fruit and Leaf Fractions
DOI:
https://doi.org/10.56919/usci.2651.041Keywords:
Antiglycation, Non-fluorescent-AGEs, Borrasus aethiopum, FTIR, ChromatographyAbstract
Non-enzymatic glycation is a reaction between aldehyde groups in reducing sugars and amino groups in proteins, resulting in advanced glycation end products (AGEs) formation. Increased accumulation of tissue AGEs has been linked to numerous diabetic complications. Borassus aethiopum (African fan palm) has been reported to have pro-apoptotic, anti-inflammatory, and antipyretic activities. This study aimed to examine the antiglycation activity of Borrasus aethiopum fruits and leaf fractions targeting non-fluorescent and non-crosslinking AGEs in vitro. Plant materials were extracted using maceration technique and then subjected to partitioning and thin-layer chromatography (TLC). Antiglycation assay was conducted using the BSA-Glucose model (using (mM) concentration) via UV-Vis detection, with aminoguanidine as the positive control. All experiments were carried out in triplicate, with data presented as mean ± standard deviation (SD), and analyzed using one-way analysis of variance (ANOVA) in SPSS version 20. The most potent fraction was characterized using attenuated total reflection-fourier transform infrared spectrometry (ATR-FTIR). Antiglycation activity revealed that the fruit chloroform fraction had the highest percentage inhibition (43% at 0 mM) compared to the leaf chloroform fraction (22%). This difference was highly significant (p < 0.05) compared to the control (59%). FTIR results showed the presence of functional groups such as alcohols, carboxylic acids, amines, carboximide, and nitro compounds, which have been reported to have therapeutic properties. Chloroform fractions of Borrasus aethiopum fruits have moderate antiglycation activity against non-fluorescent and non-crosslinking AGEs; this should be further explored to elucidate key principles for drug design and discovery.
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