Antimicrobial Properties and GC-MS Profiling of Hexane-Derived Fractions of Moringa oleifera Seed Obtained Using Ultrasonic Assisted Extraction Method
DOI:
https://doi.org/10.56919/usci.2544.017Keywords:
phytochemicals, isolate, bioactive compoundsAbstract
The Moringa oleifera seeds were sourced, identified, and extracted using ultrasonic extraction with hexane as the extraction solvent. The extract was subjected to qualitative phytochemical screening for the detection of plant metabolites using standard procedures. The isolates were collected from a tertiary hospital and were reconfirmed using standard identification procedures. The extract was fractionated and tested against the bacterial isolates; the bioactive compounds in the fractions were characterized using GC-MS. Qualitative analysis of the extract shows the presence of alkaloids, saponins, tannins, flavonoids, and phenols. Antimicrobial activity of the fractions shows broad-spectrum activity against the isolates, with mean zones of inhibition of 22 mm (butanol fraction), 22 mm (aqueous fraction), and 20.5 mm (hexane fraction). GC-MS characterization of the fractions detected key compounds like (BF oleic acid (98%), n-hexadecenoic acid (99%), Docosonoic acid (99%), Palmitoleic acid. (99%). (AF Linolaidic acid (95%), 6-octadecenoic acid (97%), cis-13- ecosenoic acid (97%), n- hexadecenoic acid (90%). (HF Phenol (87%), cis-vaccenic acid (90%), n-hexadecanoic acid (99%), 1- prophyl 9- octadecenoate heptadecanolide (95%). Conclusively, the results have revealed that the Ultrasonic Extraction method using seed and Hexane recovered phytochemicals, which significantly affected antimicrobial activity against the tested bacterial isolates.
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