Comparative Foliar Epidermal Morphology of the Genus Terminalia L. in Nigeria
DOI:
https://doi.org/10.56919/usci.2542.045Keywords:
Stomata, Anatomy, Terminalia, Light Microscopy, taxonomic significanceAbstract
Foliar epidermal features play an important role in clarifying the taxonomy and systematics of flowering plants. This study investigates leaf epidermal morphology of the eight Terminalia species represented in Nigeria using light microscopy to resolve besetting taxonomic issues. Leaf sections were macerated, cleaned, mounted, stained, and sealed on slides for microscopic examination. Stomatal index and other measurements were then recorded. The findings revealed variability in stomatal and other epidermal features among the species, although some characteristic features remained consistent. Epidermal cells generally exhibit a regular and sinuous shape, with the exception of Terminalia schimperiana, which displays isodiametric cells on both surfaces. The anticlinal cell walls are either straight or undulating; however, they are curved on the adaxial surface of Terminalia macroptera. All the species studied exhibit hypostomatic and anomocytic stomata except for Terminalia macroptera, which has amphistomatic leaves. Multangulate trichomes were recorded in Terminalia avicennioides, and falcate trichomes in Terminalia schimperiana. The stomatal indices varied significantly, ranging from 28.75% in Terminalia laxiflora to 5.95% in Terminalia macroptera. Moreover, the examined species showed significant variations in stomatal type, anticlinal wall type, epidermal cell type, and trichome presence. However, these taxonomic features have been recognized as diagnostic and highly significant characteristics for resolving taxonomic issues and aiding the accurate identification of these species for effective conservation and monitoring. An artificial taxonomic key was developed based on foliar epidermal characteristics to accurately delineate and identify the Nigerian Terminalia spp. Therefore, further anatomical research utilizing a scanning electron microscope is recommended to strengthen the systematics of Terminalia in Nigeria.
References
Adedeji, O. (2004). Leaf epidermal studies of the species of Emilia Cass. (Senecioneae, Asteraceae) in Nigeria. Botanica Lithuanica, 10(2), 121–133.
Adeniji, O. A., Zaccheaus, O. S., Ojo, B. S., & Adedeji, A. S. (2015). Charcoal production and producers' tree species preference in Borgu Local Government Area of Niger State, Nigeria. Journal of Energy Technologies and Policy, 5(11), 1–8.
Adewuyi, A. M., Akangbe, Y. T., Animashaun, D. A., Durodola, F. A., & Bello, O. B. (2015). Terminalia avicennioides as a potential candidate for pharmaceutical industry: A review. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6(2), 748–754.
Akinsulire, O. P., Oladipo, O. T., Akinkunmi, O. C., Adeleye, O. E., & Akinloye, A. J. (2020). On the systematic implication of foliar epidermal micro-morphological and venational characters: Diversities in some selected Nigerian species of Combretaceae. Acta Biologica Slovenica, 63(1), 25–43. [https://doi.org/10.14720/abs.63.1.15913] DOI: https://doi.org/10.14720/abs.63.1.15913
Akinsulire, O. P., Oladipo, O. T., Akinloye, A. J., Adeleye, O., & Illoh, H. C. (2018). Structure, distribution and taxonomic significance of leaf and petiole anatomical characters in five species of Terminalia (L.) (Combretaceae: Magnoliopsida). Brazilian Journal of Biological Sciences, 5(10), 515–528. https://doi.org/10.21472/bjbs.051027 DOI: https://doi.org/10.21472/bjbs.051027
Aliyu, H., Suleiman, M., Ahmed, A., Chiezay, N., Ahmed, A., Mamman, M., & McGaw, L. (2018). Terminalia avicennioides Guill & Perr (Combretaceae): Pharmacology and phytochemistry of an alternative traditional medicine in Nigeria: Mini review. Journal of Pharmacognosy and Natural Products, 4(2), 1–4. https://doi.org/10.4172/2472-0992.1000152 DOI: https://doi.org/10.4172/2472-0992.1000152
Bhatia, R. C. (1984). Foliar epidermal studies of Heliotropium supinum L. Folia Geobotanica et Phytotaxonomica, 19, 381–385. https://doi.org/10.1007/BF02853177 DOI: https://doi.org/10.1007/BF02853177
Burkill, H. M. (2004). The useful plants of West Tropical Africa. Royal Botanic Gardens, Kew.
Chimezie, E., & Agbagwa, I. O. (2015). Epidermal structures and stomatal ontogeny in Terminalia catappa L. (Combretaceae). International Journal of Botany, 11(1), 1–9. https://doi.org/10.3923/ijb.2015.1.9 DOI: https://doi.org/10.3923/ijb.2015.1.9
Cock, I. E. (2015). The medicinal properties and phytochemistry of plants of the genus Terminalia (Combretaceae). Inflammopharmacology, 23(5), 203–229. https://doi.org/10.1007/s10787-015-0246-z DOI: https://doi.org/10.1007/s10787-015-0246-z
Damaiyani, J., Fiqa, A. P., Rindyastuti, R., Lestari, D. A., Rahadiantoro, A., & Yulistyarini, T. (2022). Comparative anatomical study of leaves for twelve Indonesian woody plant species. Biodiversitas Journal of Biological Diversity, 23(7). https://doi.org/10.13057/biodiv/d230751 DOI: https://doi.org/10.13057/biodiv/d230751
Deshmukh, V. P., Thakare, P. V., Chaudhari, U. S., Gawande, P. A., & Undal, V. S. (2009). Assessment of genetic diversity among Terminalia species using RAPD markers. Global Journal of Biotechnology & Biochemistry, 4(2), 70–74.
Erakhrumen, A. A., Ogunsanwo, O. Y., & Ajewole, O. I. (2010). Assessment of some other traditional uses of accepted agro-forestry fuelwood species in Akinyele and Ido Local Government Areas, Oyo State, Nigeria. International Journal of Social Forestry, 3(1), 49–65.
Govaerts, R., Campacci, M. A., Baptista, D. H., Cribb, P. J., George, A., Kreuz, K., & Wood, J. (2020). World checklist of Orchidaceae. Royal Botanic Gardens, Kew. http://www.kew.org/wcsp/monocots
Hawthorne, W. (1998). Terminalia ivorensis. The IUCN Red List of Threatened Species 1998: e.T33062A9754250. https://dx.doi.org/10.2305/IUCN.UK.1998.RLTS.T33062A9754250.en DOI: https://doi.org/10.2305/IUCN.UK.1998.RLTS.T33062A9754250.en
Holmgren, P. K., Holmgren, N. H., & Barnett, L. C. (1990). Index herbariorum. Part I: The herbaria of the world (8th ed.). Regnum Vegetabile.
Hutchinson, J., & Dalziel, J. M. (1954). Flora of West Tropical Africa (Vol. 1, Parts 1 & 2).
Illoh, H. C. (1995). Foliar epidermis and petiole anatomy of four species of Celosia L. in Nigeria. Feddes Repertorium, 106(1–5), 15–23. [https://doi.org/10.1002/fedr.19951060107] DOI: https://doi.org/10.1002/fedr.19951060107
IUCN. (2014). The IUCN Red List of Threatened Species. http://www.iucnredlist.org/
Jones, J. H. (1986). Evolution of the Fagaceae: The implication of foliar features. Annals of the Missouri Botanical Garden, 73, 228–275. https://doi.org/10.2307/2399112 DOI: https://doi.org/10.2307/2399112
Kadiri, A. B., Ayodele, A. E., & Olowokudejo, J. D. (2019). Leaf epidermis of the African genus Mallotus Lour. (Euphorbiaceae) section Rottleropsis Müll. Arg. and its systematic value. Webbia. https://doi.org/10.1080/00837792.2019.1589742 DOI: https://doi.org/10.1080/00837792.2019.1589742
Kadiri, A. B., Ogundipe, O. T., & Utubor, D. (2013). Taxonomic relationship in Lagenaria siceraria (Cucurbitaceae) based on foliar epidermal morphology. Thaiszia Journal of Botany, 23(1), 47–59.
Keay, R. W. (1989). Trees of Nigeria. Clarendon Press. https://doi.org/10.1093/oso/9780198545606.001.0001 DOI: https://doi.org/10.1093/oso/9780198545606.001.0001
Khan, K., Khan, M., Ahmad, M., Shah, G. M., Zafar, M., Niamat, R., Munir, M., Abbasi, A. M., Fazal, H., Mazari, P., & Seema, N. (2011). Foliar epidermal anatomy of some ethnobotanically important species of genus Ficus Linn. Journal of Medicinal Plants Research, 5, 1627–1638.
Kimpouni, V. (2009). Terminalia superba Engl. & Diels. In R. H. M. J. Lemmens, D. Louppe, & A. A. Oteng-Amoako (Eds.), PROTA (Plant Resources of Tropical Africa/Ressources végétales de l'Afrique tropicale). Wageningen, Netherlands.
Kokou, K., Nuto, Y., & Atsri, H. (2009). Impact of charcoal production on woody plant species in West Africa: A case study in Togo. Scientific Research and Essay, 4, 881–893.
Maurin, O., Chase, O. M., Jordaan, M., & Van Der Bank, M. (2010). Phylogenetic relationships of Combretaceae inferred from nuclear and plastid DNA sequence data: Implications for generic classification. Botanical Journal of the Linnean Society, 162, 453–476. https://doi.org/10.1111/j.1095-8339.2010.01027.x DOI: https://doi.org/10.1111/j.1095-8339.2010.01027.x
Metcalfe, C. R., & Chalk, L. (1950). Anatomy of the dicotyledons. Oxford University Press.
Metcalfe, C. R., & Chalk, L. (1979). Anatomy of the dicotyledons (2nd ed., Vol. 1). Clarendon Press.
Mishra, P., Kumar, A., Nagireddy, A., Shukla, A. K., & Sundaresan, V. (2017). Evaluation of single and multilocus DNA barcodes towards species delineation in complex tree genus Terminalia. PLOS ONE, 12(8). https://doi.org/10.1371/journal.pone.0182836 DOI: https://doi.org/10.1371/journal.pone.0182836
Munir, M., Khan, M. A., Ahmed, A., Beno, A., Ahmed, K., Tariq, S., Tabassum, T., Mukhtar, M., Ambreen, M., & Bashir, S. (2011). Foliar anatomy of some ethnobotanical important species of wild edible fruits of North Pakistan. Journal of Medicinal Plants Research, 5(24), 5871–5880.
Nithaniyal, S., & Parani, M. (2016). Evaluation of chloroplast and nuclear DNA barcodes for species identification in Terminalia L. Biochemical Systematics and Ecology, 68, 223–229. https://doi.org/10.1016/j.bse.2016.08.001 DOI: https://doi.org/10.1016/j.bse.2016.08.001
Nodza, I. G., Abdulhameed, A., & Abdullahi, M. B. (2013). A checklist and ethnobotanical assessment of tree species of Abubakar Tafawa Balewa University (ATBU) Yelwa campus Bauchi, Nigeria. International Journal of Botany, 9, 55–63. https://doi.org/10.3923/ijb.2013.55.63 DOI: https://doi.org/10.3923/ijb.2013.55.63
Ogara, J. I. (2011). Preliminary studies on charcoal production and producers' knowledge of environmental hazards in Nasarawa State, Nigeria. Production Agriculture and Technology, 7(2), 68–75.
Ogundipe, O. T. (2004). Foliar micromorphology of the species of Blighia Konig (Sapindaceae) in West Africa. Nigerian Journal of Botany, 17, 53–61.
Ogundipe, O. T., & Wujek, D. E. (2004). Foliar anatomy on twelve genera of Bignoniaceae (Lamiales). Acta Botanica Hungarica, 46(3–4), 290–312. https://doi.org/10.1556/ABot.46.2004.3-4.7 DOI: https://doi.org/10.1556/ABot.46.2004.3-4.7
Ogunsanwo, O. Y., Aiyeloja, A. A., & Uzo, C. (2007). Production technique and the influence of wood species on the properties of charcoal in Nigeria, a case study of Oyo State. Agricultural Journal, 2(1), 131–133.
Onana, J. M., & Cheek, M. (2011). Red data list of the flowering plants of Cameroon: IUCN global assessments. Royal Botanic Gardens, Kew.
Onuminya, T. O., Sokunbi, M. R., & Ogundipe, O. T. (2020). Systematic significance of morphological and foliar epidermal characters in six species of Pancovia Willd (Family: Sapindaceae Juss). Feddes Repertorium, 131, 116–124. https://doi.org/10.1002/fedr.202000007 DOI: https://doi.org/10.1002/fedr.202000007
Onyekwelu, J. C., & Stimm, B. (2004). Terminalia superba. Enzyklopädie der Holzgewächse: Handbuch und Atlas der Dendrologie, 1–12.
Orimoogunje, O. I., & Asifat, J. (2015). Fuel wood consumption and species degradation in South-Western Nigeria: The ecological relevance. Journal of Landscape Ecology, 8(1), 56–68. https://doi.org/10.1515/jlecol-2015-0004 DOI: https://doi.org/10.1515/jlecol-2015-0004
Paul, P., & Chowdhury, M. (2021). Foliar micromorphology as a tool for identification of Indian taxa of Polygonaceae. Journal of Asia-Pacific Biodiversity, 14(4), 569–589. https://doi.org/10.1016/j.japb.2021.05.008 DOI: https://doi.org/10.1016/j.japb.2021.05.008
POWO. (2023). Plants of the World Online. Royal Botanic Gardens, Kew. http://www.plantsoftheworldonline.org/
Ramassamy, V., Karthikeyan, N., & Kannabiran, B. (1988). A note on the paracytic stomata of perigenous origin in Terminalia bellirica Roxb. Phytomorphology, 38(4), 343–345.
Stace, C. A. (1965). Cuticular studies as an aid to plant taxonomy. Bulletin of the British Museum (Natural History) Botany, 4, 3–78.
Stace, C. A. (1965b). The significance of the leaf epidermis in the taxonomy of the Combretaceae. Journal of the Linnean Society of London, Botany, 59, 229–252. https://doi.org/10.1111/j.1095-8339.1965.tb00060.x DOI: https://doi.org/10.1111/j.1095-8339.1965.tb00060.x
Stace, C. A. (1984). The taxonomic importance of leaf surface. In V. H. Heywood & D. M. Moore (Eds.), Current concepts in plant taxonomy (pp. 67–94). Academic Press.
Tilney, P. M. (2002). A contribution to the leaf and young stem anatomy of the Combretaceae. Botanical Journal of the Linnean Society, 138, 163–196. https://doi.org/10.1046/j.1095-8339.2002.138002163.x DOI: https://doi.org/10.1046/j.1095-8339.2002.138002163.x
Uzoechina, C. V. (1978). A taxonomic study of two closely related species: Terminalia ivorensis A. Chev. and T. glaucescens Planch. ex Benth. in Nigeria. Annals of Botany, 42(6), 1375–1381. [https://doi.org/10.1093/oxfordjournals.aob.a085584] DOI: https://doi.org/10.1093/oxfordjournals.aob.a085584
Wahua, C., & Iheaka, I. (2017). Micromorphological properties and phytochemistry constituents of Terminalia ivorensis A. Chev and Terminalia catappa Linn. International Journal of Current Research, 9(6), 52358–52361.
Waman, M. B. (2015). Leaf epidermal traits of some species of Terminalia and their taxonomic implications. International Journal of Recent Trends in Science and Technology, 15(3), 526–529. http://www.statperson.com/
Zhang, X. R., Kaunda, J. S., Zhu, H. T., Wang, D., Yang, C. R., & Zhang, Y. J. (2019). The genus Terminalia (Combretaceae): An ethnopharmacological, phytochemical and pharmacological review. Natural Products and Bioprospecting, 9(6), 357–392. https://doi.org/10.1007/s13659-019-00222-3 DOI: https://doi.org/10.1007/s13659-019-00222-3
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