Biopesticidal Activity of Balanites, Nigella, and Neem Essential Oils against Callosobruchus maculatus L. on Stored Cowpea (Vigna unguiculata L Walp) in Sudan Savanna, Nigeria
DOI:
https://doi.org/10.56919/usci.2543.043Keywords:
Essential oils, Callosobruchus maculatus, oviposition, deterrence, Sudan savannaAbstract
An experiment was conducted to assess the oviposition deterrence effect and identify bioactive compounds in some essential oils (EOs) on cowpea weevil (Callosobruchus maculatus L.) infesting stored cowpea (Vigna unguiculata L Walp). Three EOs, obtained from balanite, black seed, and neem; a synthetic insecticide; and a control were tested alongside three local cowpea cultivars (Dan Misra, Kwankwasiya, and Kananado). The treatments were applied at a rate of 2.5 mL per 50 g of cowpea (equivalent to 0.05 mL/g v/w). Treatments were arranged in a Completely Randomized Design (CRD) and were replicated three times. Data were collected on adult mortality, oviposition, and progeny emergence. Bioactive compounds with pesticidal properties were also analyzed. The data were subjected to analysis of variance (ANOVA), and significant means were separated using Duncan’s Multiple Range Test (DMRT) at 5% probability. Adult mortality was significantly (P<0.05) higher in insecticide-treated cowpeas 24 hours after infestation, followed by balanite oil. Black seed and neem oils performed similarly and were significantly more effective than the control. Complete oviposition deterrence was observed in insecticide-treated cowpeas, but the effects were not significantly different (P>0.05) from those of the EOs. Balanite oil recorded the lowest number of eggs among the EOs. Progeny emergence was significantly (P<0.05) lower in the EOs, but was comparable to the insecticide treatment and significantly different from the control. Bioactive compounds with insecticidal and repellent properties were identified using Gas Chromatography-Mass Spectrometry (GC-MS), which included tetradecane, oleic acid, octadecanoic acid, tetradecanoic acid, 2,4-di-tert-butylphenol, and tetradecanoic acid in black seed and balanite oils, respectively. Based on these findings, essential oils, particularly balanite and black seed oils, could serve as environmentally friendly alternatives to synthetic insecticides for controlling bruchids in sustainable cowpea storage in the study area.
References
Abaje, I. B., Sawa, B. A., & Ati, O. F. (2014). Climate variability and change, impacts and adaptation strategies on Dutsin-Ma Local Government Area of Katsina State. Journal of Geography and Geology, 6(2), 103–112. DOI: https://doi.org/10.5539/jgg.v6n2p103
Abu Zeid, I. E., El-Mahdy, O. M., Mohamed, A. H., & Abdelgawad, F. M. (2019). Phytochemical and GC-MS analysis of bioactive compounds from Balanites aegyptiaca. Acta Scientific Pharmaceutical Sciences, 3(8), 129–134. DOI: https://doi.org/10.31080/ASPS.2019.03.0352
Adamu, U., Yusha, M., Salisu, B., & Hussain, A. M. (2022). Phytochemical screening, antibacterial potentials and gas chromatography-mass spectrometry analysis (GC-MS) of Citrus sinensis leaves extracts. Microbes and Infectious Diseases, 3(1), 192–198.
Adegbite, A. A., Afolayan, M. O., & Osipitan, O. A. (2020). Comparative effects of essential oils on mortality and reproductive inhibition of Callosobruchus maculatus. African Journal of Pest Management, 18(3), 150–158.
Adimas, Z. T., Adimas, M. A., & Abera, B. D. (2024). Plant-based bioactive compounds for grain storage: A comprehensive review. Cogent Food & Agriculture, 10(1), 2316152. DOI: https://doi.org/10.1080/23311932.2024.2316152
African Agricultural Technology Foundation (AATF). (2014). Pod-borer resistant cowpea project partnership- developing a pod-borer resistant cowpea. http://www.aatf-africa.org/
Ahmad, R., & Yousuf, M. (2021). Evaluation of Balanites aegyptiaca oil in stored grain pest management: An eco-friendly approach. Agricultural Science Journal, 33(2), 183–191.
Ahmed, A. M., Salih, H. M., & Mahmoud, M. A. (2021). Evaluation of insecticidal properties of Balanites aegyptiaca oil against stored product pests. Journal of Stored Products Research, 94, 101935.
Akhigbe, R., Bello, H., & Ahmed, Z. (2021). Evaluation of black seed oil (Nigella sativa) as a potential biopesticide against stored-product pests. African Entomology, 29(1), 123–132.
Akami, M., Chakira, H., Andongma, A., Khaeso, K., Gbaye, O., Nicolas, N., Nukenine, E., & Niu, C. (2017). Essential oil optimizes the susceptibility of Callosobruchus maculatus and enhances the nutritional qualities of stored cowpea Vigna unguiculata. Royal Society Open Science, 4(11), 170692. DOI: https://doi.org/10.1098/rsos.170692
Akinneye, J. O., Oyeniyi, E. A., & Ebere, O. J. (2021). Biological control of Callosobruchus maculatus (F.) in stored cowpea seeds using botanical extracts. Journal of Stored Products Research, 91, 101784.
Akinola, M. O., Omoniyi, T., & Adebayo, A. A. (2020). Effect of Callosobruchus maculatus infestation on the nutritional quality of stored cowpea (Vigna unguiculata). Journal of Agricultural Science and Food Technology, 6(2), 42–50.
Albakry, Z., Karrar, E., Ahmed, I. A. M., Oz, E., Proestos, C., El Sheikha, A. F., Oz, F., Wu, G., & Wang, X. (2022). Nutritional composition and volatile compounds of black cumin (Nigella sativa L.) seed, fatty acid composition and tocopherols, polyphenols, and antioxidant activity of its essential oil. Horticulturae, 8(7), 575. DOI: https://doi.org/10.3390/horticulturae8070575
Asgarpanah, J., & Ariamanesh, A. (2021). Insecticidal properties of thymoquinone and black seed oil: Applications in agricultural pest management. Phytoparasitica, 49(4), 675–684.
Aslan, E. G., Kilıç, Y., Ak, M. M., Akkaya, A. I., & Ayvaz, A. (2024). Bioinsecticidal potential of rose essential oil (Rosa damascena Mill.) against Callosobruchus maculatus (F.). Journal of Applied Entomology. Advance online publication.
Ati, M. H., Dan-Malam, R., Muhammad, A., & Musa, M. (2024). Efficacy of some vegetable oils on cowpea weevils, Callosobruchus maculatus (Fab.) fecundity in Dutsin-Ma, Sudan savanna, Nigeria. In Proceedings of the 2nd Annual Conference of Pesticidal Plants Society (PPS) (pp. 78–83).
Baghouz, A., Bouchelta, Y., Es-Safi, I., Brahimi, R., Imtara, H., AlZain, M., Noman, O., Shahat, A., & Guemmouh, R. (2024). Biocidal activity of Ziziphora hispanica L and Satureja calamintha Scheele L essential oils against the Callosobruchus maculatus (Fabricius) pest on cowpea seeds during storage. Frontiers in Sustainable Food Systems, 8, 1329100. DOI: https://doi.org/10.3389/fsufs.2024.1329100
Ebadollahi, A., & Mahboubi, M. (2020). Review on plant essential oils and their bioactivities against insects. Insects, 11(12), 874.
Ekoja, E. E., Ortese, K. J., & Babarinde, S. A. (2022). Preservation of cowpea seeds against Callosobruchus maculatus Fabricius (Coleoptera: Chrysomelidae: Bruchinae) using selected fixed vegetable oils. Annals of Entomology, 40, 13–26.
Et-Tazy, L., Lamiri, A., Bencheqroun, S. K., Errati, H., Hashem, A., Avila-Quezada, G. D., Abd-Allah, E. F., Satrani, B., Essahli, M., & Satia, L. (2025). Exploring synergistic insecticidal effects of binary mixtures of major compounds from six essential oils against Callosobruchus maculatus. Scientific Reports, 15, 15180. DOI: https://doi.org/10.1038/s41598-025-98566-z
Ghosh, A., Rana, S., & Srivastava, C. (2021). Essential oils as alternatives to synthetic pesticides in stored grain pest management. Grain Storage Science and Technology, 27(1), 33–48.
Gupta, H., Deeksha, Urvashi, & Reddy, S. G. E. (2023). Essential oils: Insecticidal activity and detoxifying enzyme inhibition in pulse beetles. Molecules, 28(2), 492. DOI: https://doi.org/10.3390/molecules28020492
Hamisu, S., & Salisu, B. (2025). GC-MS analysis and synergistic inhibition of Staphylococcus aureus, Streptococcus pyogenes and dermatophytes by novel plant oil blends developed for skin and hair therapy. UMYU Journal of Microbiology Research (UJMR), 10(1), 284–295. DOI: https://doi.org/10.47430/ujmr.25101.028
Hamzei, M., Golizadeh, A., Hassanpour, M., Faithi, S. A. A., & Abedi, Z. (2023). Interaction between life history parameters of Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae) with physical and biochemical properties of legume species. Journal of Stored Products Research, 102, 102111. DOI: https://doi.org/10.1016/j.jspr.2023.102111
Ileke, K. D., Adesina, J. M., Nwosu, L. C., & Olagunju, A. (2020). Perforation index assessment of cowpea seeds against cowpea bruchid, Callosobruchus maculatus (Fabricius) [Coleoptera: Chrysomelidae], infestation using Piper guineense. The Journal of Basic and Applied Zoology, 81, 60. DOI: https://doi.org/10.1186/s41936-020-00195-7
Isman, M. B. (2019). Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology, 64, 19–38.
Jallow, M. F. A., Awadh, D. G., Albaho, M. S., Devi, V. Y., & Thomas, B. M. (2020). Pesticide risk behaviors and factors influencing pesticide use among farmers in Kuwait. Science of the Total Environment, 742, 140612.
Jumbo, L., Haddi, K., Faroni, L., Heleno, F., Pinto, F., & Oliveira, E. (2018). Toxicity to, oviposition and population growth impairments of Callosobruchus maculatus exposed to clove and cinnamon essential oils. PLoS ONE, 13(11), e0207618. DOI: https://doi.org/10.1371/journal.pone.0207618
Kalpna, A., Hajam, Y. A., & Kumar, R. (2022). Management of stored grain pest with special reference to Callosobruchus maculatus, a major pest of cowpea: A review. Heliyon, 8(2), e08703. DOI: https://doi.org/10.1016/j.heliyon.2021.e08703
Kareem, A. A., Ibrahim, A. M., & Usman, M. (2020). Potential of black seed oil (Nigella sativa) as a botanical insecticide in stored cowpea pest management. African Journal of Agricultural Science, 15(3), 342–350.
Kariuki, J. W., Wanjiru, W. M., & Githiri, S. M. (2021). Comparative efficacy of selected plant-based insecticides in the control of Callosobruchus maculatus on stored cowpea (Vigna unguiculata) seeds. Journal of Stored Products and Postharvest Research, 12(1), 1–8.
Kavallieratos, N. G., Boukouvala, M. C., Skourti, M., Benelli, G., & Papanikolaou, N. E. (2024). Exploring the efficacy of four Apiaceae essential oils as grain protectants against key stored-product pests. Plants, 13(4), 533. DOI: https://doi.org/10.3390/plants13040533
Kéïta, S. M., Vincent, C., Schmit, J., Ramaswamy, S., & Bélanger, A. (2000). Effect of various essential oils on Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). Journal of Stored Products Research, 36(4), 355–364. DOI: https://doi.org/10.1016/S0022-474X(99)00055-7
Khan, A. A., & Mian, M. H. (2020). Neem oil and its bioactive compounds as insect growth regulators for the management of stored-product pests. Pest Management Science, 76(4), 1186–1194.
Khan, R., Baloch, M., & Karim, F. (2021). Effectiveness of neem oil for protection of cowpea seeds against Callosobruchus maculatus. Journal of Agricultural and Food Chemistry, 69(3), 512–520.
Kumar, R., Singh, B., & Mishra, S. (2021). Economic implications of pest infestations in legume storage: A focus on Callosobruchus maculatus. Journal of Pest Science, 94(2), 203–213.
Larweh, V. D., Afun, J. V. K., Akromah, R., Amoah, S., & Kusi, F. (2025). Farmers' perceptions on causes of seed beetle infestation in stored cowpea in Ghana. African Crop Science Journal, 33(1), 57–70. DOI: https://doi.org/10.4314/acsj.v33i1.6
Malami, B., & Usman, H. (2022). Assessing black seed oil (Nigella sativa) as a pest control agent for cowpea seed bruchid (Callosobruchus maculatus). Journal of Stored Products and Postharvest Research, 13(2), 97–105.
Maurya, A., Yadav, A., Soni, M., Kumar, K. P., Bairwa, U., Jangir, M. K., & Dudi, N. K. (2024). Nanoencapsulated essential oils for post-harvest preservation of stored cereals: A review. Foods, 13(24), 4013. DOI: https://doi.org/10.3390/foods13244013
Mohammed, A. H., Na'inna, S. Z., Yusha'u, M., Salisu, B., Adamu, U., & Kabir, Z. M. (2017). Antibacterial, cytotoxicity and GC-MS analysis of Psidium guajava extracts. Bayero Journal of Pure and Applied Sciences, 10(1), 163–169. DOI: https://doi.org/10.4314/bajopas.v10i1.23
Mssillou, I., Saghrouchni, H., Saber, M., Zannou, A., Balahbib, A., Bouyahya, A., Allali, A., Lyoussi, B., & Derwich, E. (2022). Efficacy and role of essential oils as bio-insecticide against the pulse beetle Callosobruchus maculatus (F.) in post-harvest crops. Industrial Crops and Products, 188, 115786. DOI: https://doi.org/10.1016/j.indcrop.2022.115786
Muhammad, A. (2017). Callosobruchus maculatus (Fab.) control by plant products in cowpea grains under storage: A review. Journal of Medicinal Botany, 1, 51–57.
Muhammad, A., & Bashir, A. K. (2017). Callosobruchus maculatus (Fab.) control by plant products in cowpea grains under storage: A review. Journal of Medicinal Botany, 1, 51–57. DOI: https://doi.org/10.25081/jmb.2017.v1.897
Muhammad, A., & Kashere, M. A. (2020). Neem, Azadirachta indica L. (A. Juss): An eco-friendly botanical insecticide for managing farmers' insects pest problems- a review. FUDMA Journal of Sciences (FJS), 4(4), 484–491. DOI: https://doi.org/10.33003/fjs-2020-0404-506
Muhammad, A., Aminu, Z. R., Muhammad, M., Ladan, K. M., Mahmoud, B. A., Abubakar, A. A., Muhammad, L., Yusuf, L. I., & Aderolu, I. A. (2023). Effect of indigenous plant materials on cowpea seed bruchids, Callosobruchus maculatus (Fab.) development and damage on cowpea, Vigna unguiculata under storage in Dutsin-Ma, Sudan savanna. In Proceedings of the 1st Annual Conference of Pesticidal Plants Society (PPS) (pp. 123–134).
Muhammad, A., Yusuf, L. I. and Oaya, C. S. (2020). Insecticidal comparison of botanicals and synthetic Insecticide on cowpea bruchid, Callosobruchus maculatus (Fab.) [Coleoptera: Chrysomelidae] development on Vigna unguiculata (L.) Walp. Journal of Entomology, 17(2): 68-73. DOI: 10.3923/je.2020.68.73. DOI: https://doi.org/10.3923/je.2020.68.73
Muhammad, A., Malgwi, A. M., & Nahunnaro, H. (2019). Maruca vitrata (Fab.) [Lepidoptera: Pyralidae] damage on cowpea (Vigna unguiculata L. Walp.) in Katsina, Sudan Savanna, Nigeria: The role of IPM. Biochemistry and Molecular Biology, 4(6), 86–93. DOI: https://doi.org/10.11648/j.bmb.20190406.12
Muhammad, A., Musa, M., Sani, S., & Ibrahim, U. M. (2024). Environmental contamination by agrochemicals and its indiscriminate use by dry season farmers in Sudan Savannah of Katsina State. Sahel Journal of Life Sciences FUDMA, 2(1), 67–77. DOI: https://doi.org/10.33003/sajols-2024-0201-009
Ngamo, T. L., Hance, T., & Kouninki, H. (2020). Comparative efficacy of essential oils on the control of major storage pests. Tropical Pest Management, 66(3), 203–210.
Ngonadi, E. N. (2025). Efficacy of selected botanicals as storage protectant against cowpea weevil (Callosobruchus maculatus) in bambara nut (Vigna Subteraanea) storage. Malaysian Journal of Halal Research (MJHR), 8(1), 12–14.
Oaya, C. S., Malgwi, A. M., Umar, I., & Mohammed, A. (2017). Insecticidal deterrence of wild spikenard (Hyptis suaveolens L. Poit) on adult mortality and female oviposition of groundnut bruchid, Caryedon serratus Olivier on stored groundnut seeds and tamarind pods. Recent Research in Science and Technology, 9, 25–29. DOI: https://doi.org/10.25081/rrst.2017.9.3369
Oaya, C. S., Malgwi, A. M., Samaila, A. E., Wahedi, J. A., Dunuwel, D. M., & Medugu, M. A. (2023). Efficacy of Hyptis suaveolens (L.) Poit) powder against Caryedon serratus Olivier [Coleoptera: Bruchidae] on stored groundnut and tamarind under different periods of storage. In Proceedings of the 1st Annual Conference of Pesticidal Plants Society (pp. 147–158).
Osipitan, A. A., & Tijani-Eniola, H. (2020). Role of saponin-rich botanicals in pest control: Balanite aegyptiaca as a case study. Journal of Organic Agriculture and Environment, 8(4), 67–75.
Popescu, I. E., Gostin, I. N., & Blidar, C. F. (2024). An overview of the mechanisms of action and administration technologies of essential oils used as green insecticides. AgriEngineering, 6(2), 1195–1217. DOI: https://doi.org/10.3390/agriengineering6020068
Prakash, S., & Rao, N. S. (2019). Challenges in the use of chemical insecticides in the management of Callosobruchus maculatus in storage: A review. Pesticide Biochemistry and Physiology, 161, 48–56.
Prieto-Rodríguez, J. A., Patiño-Bayona, W. R., & Patiño-Ladino, O. J. (2025). Chemical composition, insecticidal and repellent activities of essential oils from Piper asperiusculum and Piper pertomentellum against red flour weevil. Records of Natural Products, 19(2), 169–181. DOI: https://doi.org/10.25135/rnp.501.2408.3279
Rattanapun, M., & Shrestha, B. (2021). The use of natural products for pest management in stored products. Natural Products and Biopesticides, 2(1), 1–9.
Raveau, R., Fontaine, J., & Lounès-Hadj Sahraoui, A. (2020). Essential oils as potential alternative biocontrol products against plant pathogens and weeds. Foods, 9(3), 365. DOI: https://doi.org/10.3390/foods9030365
Ripoll-Aristizabal, D. C., Patino-Ladino, O. J., & Prieto-Rodríguez, J. A. (2025). Essential oils and phenylpropanoids from piper: Bioactivity and enzyme inhibition in Sitophilus zeamais and Tribolium castaneum. Journal of Stored Products Research, 108, 102714. DOI: https://doi.org/10.1016/j.jspr.2025.102714
Rubasinghege, G., Paranagama, P., & Abeywickrama, K. (2006). Physicochemical changes of stored cowpea, Vigna unguiculata, treated with selected essential oils to control cowpea bruchid, Callosobruchus maculatus (F.). International Journal of Food, Agriculture and Environment, 4(2), 41–42.
Salisu, B. D., Magaji, A. M., & Abdulkadir, B. (2017). Phytochemical determination and in vitro antimicrobial activity of crude ethanolic extract of stem bark of Boswellia dalzielii. International Journal of Science and Research (IJSR), 6(12), 1484–1492. DOI: https://doi.org/10.21275/ART20178999
Salisu, B., Ibrahim, F., Kaware, M. S., & Isah, M. (2025). Gas chromatographic evaluation of hydrocarbon degradation capabilities of Phyllosphere-derived Bacteria in simulated bioremediation of contaminated soil. UMYU Journal of Microbiology Research (UJMR), 10(1), 21–31. DOI: https://doi.org/10.47430/ujmr.25101.003
Sanon, A., Zakaria, I., Dabire-Binso, C. L., Niango, B. M., & Honora, N. R. C. (2018). Potential of botanicals to control Callosobruchus maculatus (Coleoptera: Chrysomelidae, Bruchinae), a major pest of stored cowpeas in Burkina Faso: A review. International Journal of Insect Science, 10, 1–8. DOI: https://doi.org/10.1177/1179543318790260
Sarrami, S., Mohajeri, F. A., Sadeghizadeh-Yazdi, J., Jambarsang, S., & Sadrabad, E. K. (2023). Chemical composition and antioxidant activity of clove essential oil and its effect on stability of sesame oil under accelerated condition. Journal of Nutrition and Food Security (JNFS), 8(3), 343–352. DOI: https://doi.org/10.18502/jnfs.v8i3.13280
SAS Institute Inc. (2002). SAS (Version 9.0) [Computer software].
Singh, B., & Kang, M. S. (2021). Postharvest losses of cowpea grains due to insect pests and mitigation strategies. Advances in Legume Research, 18(4), 309–318.
Tchouassi, D. P., Muturi, E. J., & Soley, R. (2020). Infestation dynamics and management of Callosobruchus maculatus in tropical agriculture: Impacts on stored cowpea. Frontiers in Sustainable Food Systems, 4, 15.
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