Immunotoxicity and proliferation of human peripheral blood mononuclear cells of HIV-positive patient after treatment with aqueous extract of polyherbal formulation

Authors

  • Abubakar Babangida Usman Department of Immunology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria Author
  • Samuel Eniola Gana Department of Immunology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria Author
  • Kabir Magaji Hamid Department of Immunology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria Author
  • Nura Kaoje Abubakar Department of Immunology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria Author
  • Hamisu Abdullahi Department of Immunology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria Author
  • Sulaiman Yahaya Isah Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, P.M.B. 3011, Kano, Nigeria Author
  • Mansur Aliyu Department of Medical Microbiology and Parasitology, Faculty of Clinical Sciences, College of Health Sciences, Bayero University, P.M.B. 3011, Kano, Nigeria Author

DOI:

https://doi.org/10.56919/usci.1122.005

Keywords:

Polyherbal formulation, immunotoxicity, proliferation, peripheral blood mononuclear cells, human immunodeficiency virus

Abstract

Medicinal plants have been widely used for medicinal purposes in the treatment of various ailments including human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS). This study evaluated the effect of aqueous extracts of polyherbal formulation (PHF) on peripheral blood mononuclear cells (PBMCs) of an HIV+ patient. In this study, 4 ml of blood was collected from HIV+ patient who has not commenced antiretroviral treatment. PBMCs were isolated using the Ficoll-Paque method and counted using the trypan blue assay. The isolated PBMCs were treated with different concentrations of the PHF (25 50, 100, and 200 μg/ml). Cytotoxicity and proliferation were determined using the WST-8 assay. The percentage viability in this study was 96.74%, whereas the non-viable cell percentage was 3.26%. The lowest percentage of cytotoxicity for the PHF was 0.05% at 25 μg/ml of the extract, while the highest was 5.35% at 200 μg/ml. The mean absorbance scores of the formulation's various concentrations and the control group did not significantly differ from one another (F=0.622, df = 5, p=0.69). There was significant difference (t= -4.887, df =6, 95% CI= -0.1566 to -0.0521, p = 0.003) in mean absorbance scores between control and treatment. The polyherbal formulation exerts the lowest cytotoxicity of 0.05% and significant proliferation on the PBMCs at 25 μg/ml. The PHF has potential immunoproliferative activity with a good safety margin. An in-depth study of the bioactivity of the PHF on individual immune cells is recommended.

References

Abubakar, N. K., Hamid, K. M., Haruna, M., Abdullahi, M. I., Usman, A. B., Isah, S. Y., and Aliyu, M. (2021). Protective activity of a polyherbal formulation in cyclophosphamide-induced immunosuppression. Ethiopian Pharmaceutical Journal, 37, 33-44. https://doi.org/10.4314/epj.v37i1.3 DOI: https://doi.org/10.4314/epj.v37i1.3

Ajaiyeoba, E., and Ogbole, O. (2006). A phytotherapeutic approach to Nigerian anti-HIV and immunomodulatory drug discovery. African Journal of Medicine and Medical Sciences, 35, 71-76.

Ala, A., Olotu, B., and Ohia, C. (2018). Assessment of cytotoxicity of leaf extracts of Andrographis paniculata and Aspilia africana on murine cells in vitro. Archives of Basic Applied Medicine, 6(1), 61.

Aldbass, A., Amina, M., Al Musayeib, N. M., Bhat, R. S., Al-Rashed, S., Marraiki, N., Fahmy, R., and El-Ansary, A. (2021). Cytotoxic and anti-excitotoxic effects of selected plant and algal extracts using COMET and cell viability assays. Scientific Reports, 11(1), 1-11. https://doi.org/10.1038/s41598-021-88089-8 DOI: https://doi.org/10.1038/s41598-021-88089-8

Anywar, G., Kakudidi, E., Byamukama, R., Mukonzo, J., Schubert, A., and Oryem-Origa, H. (2020). Indigenous traditional knowledge of medicinal plants used by herbalists in treating opportunistic infections among people living with HIV/AIDS in Uganda. Journal of Ethnopharmacology, 246, 112205. https://doi.org/10.1016/j.jep.2019.112205 DOI: https://doi.org/10.1016/j.jep.2019.112205

Bhardwaj, S., Almaeen, A., Ahmed Wani, F., and Thirunavukkarasu, A. (2020). Hematologic derangements in HIV/AIDS patients and their relationship with the CD4 counts: a cross-sectional study. International Journal of Clinical and Experimental Pathology, 13(4), 756-763.

Hamid, K. M., Isah, S. Y., Kalgo, M. U., Isiyaku, A., Usman, A. B., Yeldu, M. H., Yusuf, A., Abubakar, N. K., Garba, Y. B., and Muhammad, H. Y. (2021a). Imunostimulatory activity of aqueous extract of polyherbal formulation on Th1/Th2 cytokines secretion and cell mediated immune response in rats. Saudi Journal of Medical and Pharmaceutical Sciences 10, 11. https://doi.org/10.36348/sjmps.2021.v07i01.013 DOI: https://doi.org/10.36348/sjmps.2021.v07i01.013

Hamid, K. M., Shehu, A. A., Kalgo, M. U., Isiyaku, A., Alkali, S., Muhammad, T., Usman, A. B., Isah, S. Y., and Abubakar, N. K. (2021b). Immunomodulatory activity of aqueous extract of polyherbal formulation on macrophage function and humoral immune response against SRBCs in mice. Nigerian Journal of Pure and Applied Science, 34(2), 3989-3997. https://doi.org/0.48198/njpas/21.a01 DOI: https://doi.org/10.48198/NJPAS/21.A01

Hamid, K. M., Yakubu, A., Sani, S. A., Kalgo, M. U., Isiyaku, A., Umahi, N. P. F., Garba, Y. B., Aliyu, M., Isah, S. Y., and Abubakar, U. (2021c). Immunocytotoxic effect of aqueous leaf extract of Cassia occidentalis on human peripheral blood mononuclear cells and neutrophils. UMYU Journal of Microbiology Research, 6(2), 30-36. https://doi.org/10.47430/ujmr.2162.005 DOI: https://doi.org/10.47430/ujmr.2162.005

Isah, Y. S., Yeldu, M. H., Hamid, K. M., Musa, U., and Kalgo, M. U. (2018). Effect of polyherbal formulation on neutrophil function and TDAR in Wistar albino rat. European Journal of Pharmaceutical and Medical Research, 5(12), 563-568.

Isola, O. I. (2013). The" relevance" of the african traditional medicine (alternative medicine) to health care delivery system in Nigeria. The Journal of Developing Areas 319-338. https://doi.org/10.1353/jda.2013.0004 DOI: https://doi.org/10.1353/jda.2013.0004

Kalgo, M. U., Hamid, K. M., Muhammad, U. A., Balarabe, A., Yeldu, M. H., Yahaya, I. S., Kalgo, Z. M., Aliyu, B., and Bala, Y. G. (2019). Effects of aqueous stem bark extract of Vitellaria paradoxa on human neutrophil function and viability. Internaional Journal of Biological and Medical Research, 10(3), 6782-6787.

Kalgo, M. U., Hamid, K. M., Balarabe, A., Muhammad, U. A., Bala, Y. G., Raji, H. Y., Yusuf, A., Isiyaku, A., Bagudo, I. A., and Usman, A. (2020). Cytotoxic effect of aqueous stem bark extract of Vitellaria paradoxa on human peripheral blood mononuclear cells. IOSR Journal of Dental and Medical Sciences, 12(2), 48-52.

Kankara, S. S., Ibrahim, M. H., Mustafa, M., and Go, R. (2015). Ethnobotanical survey of medicinal plants used for traditional maternal healthcare in Katsina state, Nigeria. South African Journal of Botany, 97, 165-175. https://doi.org/10.1016/j.sajb.2015.01.007 DOI: https://doi.org/10.1016/j.sajb.2015.01.007

Karole, S., Shrivastava, S., Thomas, S., Soni, B., Khan, S., Dubey, J., Dubey, S. P., Khan, N., and Jain, D. K. (2019). Polyherbal formulation concept for synergic action: a review. Journal of Drug Delivery and Therapeutics, 9(1-s), 453-466. https://doi.org/10.22270/jddt.v9i1-s.2339 DOI: https://doi.org/10.22270/jddt.v9i1-s.2339

Kim, J. S., Nam, M. H., An, S. S. A., Lim, C. S., Hur, D. S., Chung, C., and Chang, J. K. (2011). Comparison of the automated fluorescence microscopic viability test with the conventional and flow cytometry methods. Journal of Clinical Laboratory Analysis, 25(2), 90-94. https://doi.org/10.1002/jcla.20438 DOI: https://doi.org/10.1002/jcla.20438

Lutoti, S. (2020). An appraisal of plants as a natural source of conventional drugs. Journal of Pharmacology and Drug Research, 3(3), 366-372.

Makgatho, M. E., Mashele, S., Makhubele, T., Sedibane, M., Bagla, V., Mashinya, F., Mokgotho, M., Masoko, P., Makola, R., and Matsebatlela, T. (2015). In vitro immune-modulatory potential of crude extract of leaf of Albizia gummifera against stimulated peripheral blood mononuclear and raw cells. African Journal of Traditional, Complementary and Alternative Medicines, 12(3), 85-90. https://doi.org/10.4314/ajtcam.v12i3.10 DOI: https://doi.org/10.4314/ajtcam.v12i3.10

McGaw, L. J., Elgorashi, E. E., and Eloff, J. N. (2014). Cytotoxicity of African medicinal plants against normal animal and human cells. In Toxicological Survey of African Medicinal Plants (pp. 181-233): Elsevier. https://doi.org/10.1016/B978-0-12-800018-2.00008-X DOI: https://doi.org/10.1016/B978-0-12-800018-2.00008-X

Mukhtar, M., Arshad, M., Ahmad, M., Pomerantz, R. J., Wigdahl, B., and Parveen, Z. (2008). Antiviral potentials of medicinal plants. Virus Research, 131(2), 111-120. https://doi.org/10.1016/j.virusres.2007.09.008 DOI: https://doi.org/10.1016/j.virusres.2007.09.008

Mussarat, S., Abdel-Salam, N. M., Tariq, A., Wazir, S. M., Ullah, R., and Adnan, M. (2014). Use of ethnomedicinal plants by the people living around Indus River. Evidence-Based Complementary and Alternative Medicine, 2014. https://doi.org/10.1155/2014/212634 DOI: https://doi.org/10.1155/2014/212634

Muthu, C., Ayyanar, M., Raja, N., and Ignacimuthu, S. (2006). Medicinal plants used by traditional healers in Kancheepuram District of Tamil Nadu, India. Journal of Ethnobiology Ethnomedicine, 2(1), 1-10. https://doi.org/10.1186/1746-4269-2-43 DOI: https://doi.org/10.1186/1746-4269-2-43

Nemudzivhadi, V., and Masoko, P. (2014). In vitro assessment of cytotoxicity, antioxidant, and anti-inflammatory activities of Ricinus communis (Euphorbiaceae) leaf extracts. Evidence-Based Complementary and Alternat Medicine, 2014, 625961. https://doi.org/10.1155/2014/625961 DOI: https://doi.org/10.1155/2014/625961

Nguyen, T. T. M., and Le, N. T. T. (2021). Inhibitory effect of medicinal plants on in vitro human peripheral blood mononuclear cell proliferation, interleukin-2 secretion and antioxidant activity. MedPharmRes, 5(1), 22-29. https://doi.org/10.32895/UMP.MPR.5.1.5 DOI: https://doi.org/10.32895/UMP.MPR.5.1.5

Nhu, T. Q., Hang, B. T. B., Vinikas, A., Hue, B. T. B., Quetin-Leclercq, J., Scippo, M.-L., Phuong, N. T., and Kestemont, P. (2019). Screening of immuno-modulatory potential of different herbal plant extracts using striped catfish (Pangasianodon hypophthalmus) leukocyte-based in vitro tests. Fish & Shellfish Immunology, 93, 296-307. https://doi.org/10.1016/j.fsi.2019.07.064 DOI: https://doi.org/10.1016/j.fsi.2019.07.064

Norisugi, O., Yoshihisa, Y., Shimizu, K., and Shimizu, T. (2014). In vitro cytokine expression by peripheral mononuclear cells in herbal drug-induced skin eruption. Acta Dermato-Venereologica, 94(1). https://doi.org/10.2340/00015555-1631 DOI: https://doi.org/10.2340/00015555-1631

Petrovska, B. B. (2012). Historical review of medicinal plants’ usage. Pharmacognosy Reviews, 6(11), 1. https://doi.org/10.4103/0973-7847.95849 DOI: https://doi.org/10.4103/0973-7847.95849

Pourahmad, J., and Salimi, A. (2015). Isolated human peripheral blood mononuclear cell (PBMC), a cost effective tool for predicting immunosuppressive effects of drugs and xenobiotics. Iranian Journal of Pharmaceutical Research, 14(4), 979.

Rickham, P. (1964). Human experimentation. Code of ethics of the world medical association. Declaration of Helsinki. British Medical Journal, 2(5402), 177-177. https://doi.org/10.1136/bmj.2.5402.177 DOI: https://doi.org/10.1136/bmj.2.5402.177

Sudeep, N., Nithya, M., and Kiranmayee, P. (2017). Evaluation of in vitro cytotoxic effects of three medicinal plants on peripheral blood mononuclear cells (PBMC). Journal of Chemical and Pharmaceutical Research 9, 18-26.

Swee, K. Y., Noorjahan, B. M. A., Wan, Y. H., Abdul, R. O., Abdul, M. A., Boon, K. B., and Abdul, H. N. Y. (2011). Immunomodulatory role of Rhaphidophora korthalsii methanol extract on human peripheral blood mononuclear cell proliferation, cytokine secretion and cytolytic activity. Journal of Medicinal Plants Research, 5(6), 958-965.

Wang, M., Raza, S. H. A., Wu, Q., Xue, C., Liu, J., Zhang, L., Zhang, W., Wang, A., and Wu, H. (2020). Cichoric acid from extracted Echinacea purpurea induces the proliferation and apoptosis of peripheral blood mononuclear cells from yaks. Electronic Journal of Biotechnology, 47, 17-28. https://doi.org/10.1016/j.ejbt.2020.06.003 DOI: https://doi.org/10.1016/j.ejbt.2020.06.003

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Published

2022-09-30

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Vol. 1 No. 1 (2022)

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How to Cite

Usman, A. B., Gana, S. E., Hamid, K. M., Abubakar, N. K., Abdullahi, H., Isah, S. Y., & Aliyu, M. (2022). Immunotoxicity and proliferation of human peripheral blood mononuclear cells of HIV-positive patient after treatment with aqueous extract of polyherbal formulation. UMYU Scientifica, 1(1), 30-35. https://doi.org/10.56919/usci.1122.005

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