Chromatographic and Spectroscopic Characterization and Identification of Compounds in Flavonoid-Rich Fraction of Thesium viride A.W.Hill
DOI:
https://doi.org/10.56919/usci.2542.029Keywords:
Thesium viride, Flavonoids, Flavonol, 1-heptacosanol, QuercetinAbstract
Thesium viride A.W.Hill is a hemi-parasite subshrub from the Santalaceae family. The aerial part of the plant is utilised in the treatment of jaundice, liver enlargement, splenomegaly, and ulcers. It is been reported that the ethyl acetate (flavonoid-rich) fraction has stronger antibacterial and antioxidant properties than the 70% ethanol extract and other fractions. This research aims to develop a chromatographic fingerprint and isolate bioactive compounds from the flavonoid-rich fraction of T. viride that may be responsible for its activity. The plant's aerial portion was collected, allowed to air dry in the shade, and then reduced in size into powder. It was macerated with 70% ethanol, and subsequently portioned using n-hexane, ethyl acetate, and n-butanol, leaving an aqueous residue. Fourier-transform infrared (FTIR), ultraviolet (UV) spectroscopy, and column chromatography were performed on the flavonoid-rich fraction, resulting in the isolation of two constituents, which were subjected to nuclear magnetic resonance (NMR) experiments for complete elucidation of their chemical structures. The UV and FTIR fingerprint profile of the flavonoid-rich fraction has been developed and can be used for identification and quality control of the fraction of T. viride. Two chemical compounds were first reported to be isolated from the flavonoid-rich fraction and characterized as 1-heptacosanol and Quercetin. The chromatographic and spectroscopic fingerprint serves as a quality control for the standardization of the flavonoid-rich fraction of T. viride. These compounds isolated may be responsible for the antimicrobial, antiulcer, antioxidant, and hepatoprotective activities of T. viride.
References
Abdi, B., Getaneh, E., Assefa, T., Dekebo, A., Tesso, H., Abdo, T., & Melaku, Y. (2020). Chemical Constituents of the Roots Extract of Dryopteris schimperiana and Evaluation for Antibacterial and Radical Scavenging Activities. Ethiopian Journal of Science and Sustainable Development, 7(1), 1–8. https://doi.org/10.20372/ejssdastu:v7.i1.2020.153
Anburaj, R., & Jothiprakasam, V. (2017). Bioassays of tannin rich fraction and identification of compounds using UV-Vis, FTIR and RP-HPLC. Research Journal of Pharmaceutical, Biological and Chemical Science, 8(1), 432–445.
Ashenafi, E. L., Nyman, M. C., Shelley, J. T., & Mattson, N. S. (2023). Spectral properties and stability of selected carotenoid and chlorophyll compounds in different solvent systems. Food Chemistry Advances, 2(2023), 100178. https://doi.org/10.1016/j.focha.2022.100178
Cobzac, S. C. A., Casoni, D., Babea, M., Balabanova, B., & Ruzdik, N. M. (2019). Ultraviolet-visible (Uv-vis) spectroscopy and cluster analysis as a rapid tool for classification of medicinal plants. Studia Universitatis Babes-Bolyai Chemia, 64(4), 191–203. https://doi.org/10.24193/subbchem.2019.4.14
Donkor, S., Larbie, C., Komlaga, G., & Emikpe, B. O. (2019). Phytochemical, Antimicrobial, and Antioxidant Profiles of Duranta erecta L. Parts. Biochemistry Research International, 2019(1). https://doi.org/10.1155/2019/8731595
Fathi, M., & Ghane, M. (2022). Phytochemical Composition, Antibacterial, and Antibiofilm Activity of Malva sylvestris Against Human Pathogenic Bacteria. Jundishapur Journal of Natural Pharmaceutical Products, 17(1), 1–9. https://doi.org/10.5812/jjnpp.114164.Research
Gololo, S. S., Mapfumari, N. S., Sethoga, L. S., Olivier, M. T., Shai, L. J., & Mogale, M. A. (2016). Identification of Phytochemical Constituents within the N-Hexane Leaf Extract of Senna italica (Mill) using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Journal of Pharmaceutical Sciences and Research, 8(10), 1141–1143.
Guemari, F., Laouini, S. E., Rebiai, A., Bouafia, A., & Barhoum, A. (2022). UV-Visible Spectroscopic Technique-Data Mining Tool as a Reliable, Fast, and Cost-Effective Method for the Prediction of Total Polyphenol Contents: Validation in a Bunch of Medicinal Plant Extracts. Applied Sciences, 12, 9430. https://doi.org/10.3390/app12199430
Inikpi, E., Lawal, O. A., Ogunmoye, A. O., & Ogunwande, A. (2014). Volatile composition of the floral essential oil of Hibiscus sabdariffa L. from Nigeria. American Journal of Essential Oils and Natural Products, 2(2), 4–7.
Iwu, M. M. (2014). Handbook of African medicinal plants (2nd ed.). https://doi.org/10.1201/b16292
Jain, P. K., Soni, A., Jain, P., & Bhawsar, J. (2016). Research Article Phytochemical analysis of Mentha spicata plant extract using UV-VIS, FTIR and GC/MS technique. Journal of Chemical and Pharmaceutical Research, 8(2), 1–6.
Jang, Y., Kim, D., Han, E., & Jung, J. (2019). Physiological Activities of Policosanol Extracted from Sugarcane Wax. Natural Product Sciences, 25(4), 293–297. https://doi.org/10.20307/nps.2019.25.4.293
Jiménez-Arellanes, M. A., Gutiérrez-Rebolledo, G. A., Meckes-Fischer, M., & León-Díaz, R. (2016). Medical plant extracts and natural compounds with a hepatoprotective effect against damage caused by antitubercular drugs: A review. Asian Pacific Journal of Tropical Medicine, 9(12), 1141–1149. https://doi.org/10.1016/j.apjtm.2016.10.010
Karpagasundari, C., & Kulothungan, S. (2014). Analysis of bioactive compounds in Physalis minima leaves using GC MS, HPLC, UV-VIS and FTIR techniques. Journal of Pharmacognosy and Phytochemistry, 3(4), 196–201.
Kaur, J., Dhiman, V., Bhadada, S., Katare, O. P., & Ghoshal, G. (2022). LC/MS guided identification of metabolites of different extracts of Cissus quadrangularis. Food Chemistry Advances, 1, 100084. https://doi.org/10.1016/j.focha.2022.100084
Kumar, S., & Pandey, A. K. (2013). Chemistry and Biological Activities of Flavonoids: An Overview. The Scientific World Journal, 2013(1). https://doi.org/10.1155/2013/162750
Kutelu, A. M., & Okwute, S. K. (2019). Phytochemical Analysis, Volatile Components and Biological Evaluation of the leaf of Calotropis procera (Asclepiadaceace). International Journal of Research and Innovation in Applied Science, 6(12), 60–67.
Leelavathi, V., & Udayasri, P. (2018). Qualitative and Quantitative Analytical Studies for the Screening of Phytochemicals from the Leaf Extracts of Senna alexandrina Mill. International Journal of Pharmaceutical and Clinical Research, 10(8), 210–215. https://www.ijpcr.com/
Liu, H., Mou, Y., Zhao, J., Wang, J., Zhou, L., Wang, M., Wang, D., Han, J., Yu, Z., & Yang, F. (2010). Flavonoids from Halostachys caspica and Their Antimicrobial and Antioxidant Activities. Molecules, 15, 7933–7945. https://doi.org/10.3390/molecules15117933
Lombard, N., Stander, M. A., Redelinghuys, H., Roux, M. M. Le, & Wyk, B. Van. (2022). A Study of Phenolic Compounds and Their Chemophenetic Value in the Genus Thesium (Santalaceae). Diversity, 14, 590, 1–29. https://doi.org/10.3390/d14080590
Mabry, T. J., Markham, K. R., & Thomas, M. B. (1970). The Ultraviolet Spectra of Flavones and Flavonols. The Systematic Identification of Flavonoids, see 111, 41–164. https://doi.org/10.1007/978-3-642-88458-0_5
Madeleine, R., Etame, E., Mouokeu, R. S., Stève, F., Poundeu, M., Voukeng, I. K., Laurel, C., Cidjeu, P., Tiabou, A. T., Joel, A., Yaya, G., Anne, R., Ngane, N., Kuiate, J. R., & Etoa, F. X. (2019). Effect of fractioning on antibacterial activity of n-butanol fraction from Enantia chlorantha stem bark methanol extract. BMC Complementary and Alternative Medicine, 19(56), 1–7. https://doi.org/10.1186/s12906-019-2459-y
Mustapha, F. J., Ella, E. E., Luka, S. A., & Wada, Y. A. (2023). Phytochemical Constituents and GC-MS Profiling of the Whole Plant Ethanol Extract of Thesium viride Hill and its Oral Toxicity in Balb/C Mouse Model. UMYU Journal of Microbiology Research, 8(1), 31–38. https://doi.org/10.47430/ujmr.2381.003
Patle, T. K., Shrivas, K., Kurrey, R., Upadhyay, S., Jangde, R., & Chauhan, R. (2020). Phytochemical screening and determination of phenolics and flavonoids in Dillenia pentagyna using UV–vis and FTIR spectroscopy. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 242, 118717. https://doi.org/10.1016/j.saa.2020.118717
Rhetso, T., Shubharani, R., Roopa, M. S., & Sivaram, V. (2020). Chemical constituents, antioxidant, and antimicrobial activity of Allium chinense G. Future Journal of Pharmaceutical Sciences, 6(102). https://doi.org/10.1186/s43094-020-00100-7
Sánchez-Hernández, E., Buzón-Durán, L., Lorenzo-Vidal, B., Martín-Gil, J. M., & Martín-Ramos, P. (2021). Physicochemical Characterization and Antimicrobial Activity against Erwinia amylovora, Erwinia vitivora, and Diplodia seriata of a Light Purple Hibiscus syriacus L. Cultivar. https://doi.org/10.3390/plants10091876
Shehu, S., Ibrahim, G., Iliyasu, U., Shehu, S., Nuhu, A., & Abubakar, M. (2017). Evaluation of antiulcer activity of aqueous ethanol extract of Thesium viride on ethanol and aspirin induced models in rats. Bayero Journal of Pure and Applied Sciences, 9(2), 82. https://doi.org/10.4314/bajopas.v9i2.16
Shehu, S., & Iliyasu, U. (2017). Pharmacognostic and Physicochemical Analysis of Thesium viride Hill. International Journal of Science for Global Sustainability, 4(2). https://doi.org/10.57233/ijsgs.v4i2.185
Shehu, S., Abubakar, M., Ibrahim, G., & Iliyasu, U. (2016a). Phytochemical and Antibacterial Studies on Aqueous Ethanol Extract of Thesium viride (Santalaceae). British Journal of Pharmaceutical Research, 11(2), 1–6. https://doi.org/10.9734/bjpr/2016/23046
Shehu, S., Ibrahim, G., Danmalam, U., October, N., Halilu, M., & Abubakar, M. (2016b). Isolation of Kaempferide and Antimicrobial Activity of Fractions of. African Journal of Pharmaceutical Research & Development, 8(1).
Shehu, S., Abdurahman, E. M., Danmalam, U. H., Mohammed, A., & Barau, A. I. (2022a). Evaluation of Antioxidant Activity of Thesium. Nigerian Journal of Pharmacy, 56(2), 276–284. https://doi.org/10.51412/psnnjp.2022.30
Shehu, S., Abdurahman, E. M., Danmalam, U. H., Mohammed, A., Shehu, S., & Iliyasu, U. (2022b). Effects of Thesium viride Extract and Fractions on Some Liver Biochemical Parameters in CCl₄-Induced Damage in Wistar Rats. Tropical Journal of Natural Product Research, 6(6), 1004–1009. https://doi.org/10.26538/tjnpr/v6i6.30
Tsimogiannis, D., Samiotaki, M., Panayotou, G., & Oreopoulou, V. (2007). Characterization of flavonoid subgroups and hydroxy substitution by HPLC-MS/MS. Molecules, 12(3), 593–606. https://doi.org/10.3390/12030593
Vambe, M., Aremu, A. O., Chukwujekwu, J. C., Gruz, J., Luterov, A., Finnie, F., & Van Staden, J. (2020). Antibacterial, Mutagenic Properties and Chemical Characterisation of Sugar Bush (Protea caffra Meisn.): A South African Native Shrub Species. Plants, 9(1331), 1–12. https://doi.org/10.3390/plants9101331
Yang, H., Zhou, M., Li, H., Wei, T., Tang, C., Zhou, Y., & Long, X. (2020). Effects of Low-level Lipid Peroxidation on the Permeability of Nitroaromatic Molecules across a Membrane: A Computational Study. ACS Omega, 5(10), 4798–4806. https://doi.org/10.1021/acsomega.9b03462
Yen, G. C., & Chen, H. Y. (1995). Antioxidant Activity of Various Tea Extracts in Relation to Their Antimutagenicity. Journal of Agricultural and Food Chemistry, 43(1), 27–32. https://doi.org/10.1021/jf00049a007
Zhigila, D. A., Verboom, G. A., & Muasya, A. M. (2020). An infrageneric classification of Thesium (Santalaceae) based on molecular phylogenetic data. Taxon, 6(1), 1–24. https://doi.org/10.1002/tax.12202
Zuchowski, J., Pecio, Ł., & Stochmal, A. (2014). Novel flavonol glycosides from the aerial parts of lentil (Lens culinaris). Molecules, 19(11), 18152–18178. https://doi.org/10.3390/molecules191118152
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