Assessment of Antimicrobial Resistance Trends in Listeria monocytogenes in Borno State: Implications for Food Safety and Public Health
DOI:
https://doi.org/10.47430/ujmr.25102.001Abstract
The study examines Listeria monocytogenes, a critical food-borne pathogen responsible for severe infections known as listeriosis. The research highlights the pathogen's resilience and the rising issue of antimicrobial resistance, posing a significant threat to global public health. Isolation was conducted using modified Public Health England protocols, with biochemical confirmation tests, including Gram staining and carbohydrate fermentation tests. Antimicrobial susceptibility testing was employed using the Kirby-Bauer disk diffusion method on Mueller-Hinton agar. A total of 258 samples were collected for this study, spanning diverse food types and market locations, with 8 (3.1%) samples being positive for Listeria monocytogenes. The isolates demonstrated 8(100%) sensitivity to Ciprofloxacin and Erythromycin, indicating their efficacy in treatment. However, the highest resistance was seen with Norfloxacin (2, 23.0%), followed by β-lactams (Amoxacillin and Ampiclox), as well as Streptomycin, with resistance rates reaching up to 12.5%. The study recommends implementing strict antibiotic stewardship programs, enhancing food safety measures, and conducting regular surveillance of L. monocytogenes in food and environmental samples. It establishes a foundational database for resistance trends in the study region, aiding future epidemiological and intervention strategies. A modification (a serial dilution) before plating is sometimes needed, especially in developing countries, due to a poor level of hygiene practice.
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