Proximate Analysis of Traditional Nigerian Dairy Matrices (Raw Cow Milk, Kindirmo, Nono, And Yoghurt) Obtained from Sabongari and Zaria, Kaduna State, Nigeria
DOI:
https://doi.org/10.47430/ujmr.26112.019Keywords:
Composite, Atwater, AOACAbstract
Traditional fermented dairy foods (Kindirmo, Nono, yoghurt) are widely consumed in northern Nigeria but lack systematic compositional characterization needed for microbial biotechnology. We analyzed the proximate composition and gross energy of raw cow milk and three fermented derivatives collected in Sabongari and Zaria, Kaduna State (n = 100 composite samples per product; 400 composite samples total) using AOAC reference methods. Moisture, protein, fat, ash, carbohydrate (by difference), total solids (TS), and total solids non‑fat (TSNF) were determined; energy was estimated using Atwater factors. Data (mean ± SD) show significant heterogeneity across matrices (one‑way ANOVA, p < 0.05). Kindirmo was protein‑ and fat‑enriched (protein 10.86 ± 0.71%; fat 22.04 ± 1.41%) with the highest energy density (252.52 ± 12.85 kcal/100 g). Nono was carbohydrate‑driven (carbohydrate 4.21 ± 1.27%) while yogurt exhibited intermediate, balanced nutrient fractions (energy 127.71 ± 13.79 kcal/100 g). These proximate architectures suggest distinct niches for lactic acid bacteria and different potentials for probiotic delivery and starter culture design. We provide methodological details and recommend targeted microbial analyses to complement compositional data. These results should be interpreted as compositional baselines specific to Kaduna State, rather than exhaustive representations of all traditional dairy systems in Nigeria.
References
Abdulrahman, F. A., & Sanmi, E. (2021). Physicochemical Properties, Proximate Composition and Total Viable Counts of Staphylococcus aureus in ‘Nono‘and Yogurt Samples in Kaduna, Nigeria. Turkish Journal of Agriculture - Food Science and Technology, 9(1), 15–20. DOI: https://doi.org/10.24925/turjaf.v9i1.15-20.3391
Adesemoye, E. T., Sanni, A. I., Spano, G., Capozzi, V., & Fragasso, M. (2025). Lactic acid bacteria diversity in fermented foods as potential bio-resources contributing to alleviating malnutrition in developing countries: Nigeria as a case study. Fermentation, 11(2), 103. https://www.mdpi.com/2311-5637/11/2/103 DOI: https://doi.org/10.3390/fermentation11020103
Agyei, D., Owusu-Kwarteng, J., Akabanda, F., & Akomea-Frempong, S. (2020). Indigenous African fermented dairy products: Processing technology, microbiology and health benefits. Critical reviews in food science and nutrition, 60(6), 991-1006. DOI: https://doi.org/10.1080/10408398.2018.1555133
Akano, O. I., Oderinde, F. O., & Omotayo, A. O. (2023). Agricultural yield, food nutrition, and dietary energy supply in Nigeria: Evidence from nationally representative data. Journal of Agriculture and Food Research, 11, 100525. DOI: https://doi.org/10.1016/j.jafr.2023.100525
AOAC International. (2019). Official methods of analysis of AOAC International (21st ed.).
Asefa, Z., Tesfaye, A., Desalegn, A., Daba, T., & Haile, T. (2025). Formulation and evaluation of probiotic starter culture: impact on Ethiopian cottage cheese “Ayib” safety, stability, sensory acceptability and antioxidant potential. One Health Outlook, 7(1), 17. DOI: https://doi.org/10.1186/s42522-025-00138-8
Binda, S., Hill, C., Johansen, E., Obis, D., Pot, B., Sanders, M.E., Tremblay, A., and Ouwehand, A.C. (2020). Criteria to qualify microorganisms as “probiotic” in foods and dietary supplements. Frontiers in microbiology, 11, 1662. DOI: https://doi.org/10.3389/fmicb.2020.01662
Chen, X., Rubab, M., Shen, S., Shao, Z., Ding, T., & Li, Z. (2026). Recent Advances in Core Functional Microorganisms in Fermented Foods. Food Quality and Safety, fyag041. DOI: https://doi.org/10.1093/fqsafe/fyag041
Desta, D. T., Kelikay, G. N., Zekwos, M., Eshete, M., Reda, H. H., Alemayehu, F. R., & Zula, A. T. (2021). Influence of fermentation time on proximate composition and microbial loads of Enset,(Ensete ventricosum), sampled from two different agroecological districts. Food Science & Nutrition, 9(10), 5641-5647. DOI: https://doi.org/10.1002/fsn3.2527
Egwaikhide, P. A., Malu, P. S., Lawal, U., Adelagun, R. O., & Andrew, C. (2014). Physico-chemical and microbiological analysis of fermented cow milk (Nono) consumed within Kaduna Town, North-Western Nigeria. Food Science and Quality Management, 29, 44-48. https://www.iiste.org/Journals/index.php/FSQM/article/download/14167/14475
Egwim, E., Nebechukwu, P., Ojo, O., Akpakpan, E., Onifade, S., & Akan, O. (2022). Probiotics from Selected Nigerian Fermented Foods: A Promising Source of Starter Culture for Industrial Yogurt Production. SSRN preprint. https://ssrn.com/abstract=4003165 or DOI: https://doi.org/10.2139/ssrn.4003165
Fagbemigun, O., Cho, G.S., Rösch, N., Brinks, E., Schrader, K., Bockelmann, W., Oguntoyinbo, F.A., Franz, C.M. (2021). Isolation and characterization of potential starter cultures from the Nigerian fermented milk product nono. Microorganisms, 9(3), 640. DOI: https://doi.org/10.3390/microorganisms9030640
Food and Agriculture Organization & World Health Organization. (2002). Guidelines for the evaluation of probiotics in food: Report of a Joint FAO/WHO working group on drafting guidelines for the evaluation of probiotics in food. World Health Organization. https://isappscience.org/wp-content/uploads/2019/04/probiotic_guidelines.pdf
Ganguly, S., Sabikhi, L., & Singh, A. K. (2022). Effect of probiotic fermentation on physico-chemical and nutritional parameters of milk-cereal based composite substrate. Journal of food science and technology, 59(8), 3073-3085. DOI: https://doi.org/10.1007/s13197-021-05350-8
Grujović, M. Ž., Semedo-Lemsaddek, T., & Marković, K. G. (2025). Application of probiotics in foods: A comprehensive review of benefits, challenges, and future perspectives. Foods, 14(17), 3088. DOI: https://doi.org/10.3390/foods14173088
Gwandu, S. H., Nonga, H. E., Mdegela, R. H., Katakweba, A. S., Suleiman, T. S., & Ryoba, R. (2018). Assessment of raw cow milk quality in smallholder dairy farms in Pemba Island Zanzibar, Tanzania. Veterinary Medicine International, 2018(1), 1031726. DOI: https://doi.org/10.1155/2018/1031726
Ikele, O. M., Ogu, C. T., Jiang, X., & Cavender, G. A. (2024). Evaluation of different lactic acid bacteria as starter cultures for Nono—A West African fermented dairy product. Foods, 13(19), 3030. DOI: https://doi.org/10.3390/foods13193030
Inusa, S. K., & Hassan, M. (2024). Chemical and microbiological properties of Nono (locally fermented milk) sold at Bayero University, Kano. Proceedings of the 49th Nigerian Society for Animal Production Conference, University of Ibadan. https://www.researchgate.net/publication/386390352_CHEMICAL_AND_MICROBIOLOGICAL_PROPERTIES_OF_NONO_LOCALLY_FERMENTED_MILK_SOLD_AT_BAYERO_UNIVERSITY_KANO DOI: https://doi.org/10.51791/njap.vi.5593
Jans, C., Meile, L., Kaindi, D.W.M., Kogi-Makau, W., Lamuka, P., Renault, P., Kreikemeyer, B., Lacroix, C., Hattendorf, J., Zinsstag, J., and Schelling, E. (2017) African fermented dairy products–overview of predominant technologically important microorganisms focusing on African Streptococcus infantarius variants and potential future applications for enhanced food safety and security. International Journal of Food Microbiology, 250, pp.27-36. DOI: https://doi.org/10.1016/j.ijfoodmicro.2017.03.012
John, R. S., Mai, H. M., & Kalla, D. J. U. (2024). Evaluation Of Bacterial Count In Milk Products (Tsala, Madara And Kindirmo) In Jos Metropolis, Nigeria. Nigerian Journal of Agriculture and Agricultural Technology, 4(4A), 93-100. DOI: https://doi.org/10.59331/njaat.v4i4A.863
Karssa, T. H., Kussaga, J. B., Semedo-Lemsaddek, T., & Mugula, J. K. (2025). Probiotics in fermented milk products and associated health benefits—a review. International Journal of Food Science and Technology, vvaf227. DOI: https://doi.org/10.1093/ijfood/vvaf227
Mani-López, E., Hernández-Figueroa, R. H., López-Malo, A., & Morales-Camacho, J. I. (2024). Viability and functional impact of probiotic and starter cultures in salami-type fermented meat products. Frontiers in chemistry, 12, 1507370. DOI: https://doi.org/10.3389/fchem.2024.1507370
Murata, R., Shim, Y. Y., Kim, Y. J., Reaney, M. J., & Tse, T. J. (2025). Exploring lactic acid bacteria in food, human health, and agriculture. Critical Reviews in Food Science and Nutrition, 65(32), 8122-8148. DOI: https://doi.org/10.1080/10408398.2025.2493236
Obafemi, Y. D., Oranusi, S. U., Ajanaku, K. O., Akinduti, P. A., Leech, J., & Cotter, P. D. (2022). African fermented foods: overview, emerging benefits, and novel approaches to microbiome profiling. npj Science of Food, 6(1), 15. DOI: https://doi.org/10.1038/s41538-022-00130-w
Obioha, P.I., Ouoba, L.I.I., Anyogu, A., Awamaria, B., Atchia, S., Ojimelukwe, P.C., Sutherland, J.P., and Ghoddusi, H.B. (2021). Identification and characterisation of the lactic acid bacteria associated with the traditional fermentation of dairy fermented product. Brazilian journal of microbiology, 52(2), pp.869-881. DOI: https://doi.org/10.1007/s42770-021-00461-y
Odetokun, I.A., Adetona, M.A., Ade-Yusuf, R.O., Adewoye, A.O., Ahmed, A.N., Ghali-Mohammed, I., Al-Mustapha, A.I., and Fetsch, A. (2023). Staphylococcus aureus contamination of animal-derived foods in Nigeria: a systematic review, 2002—2022. Food safety and risk, 10(1), 6. DOI: https://doi.org/10.1186/s40550-023-00106-y
Owusu-Kwarteng, J., Akabanda, F., Agyei, D., & Jespersen, L. (2020). Microbial safety of milk production and fermented dairy products in Africa. Microorganisms, 8(5), 752. DOI: https://doi.org/10.3390/microorganisms8050752
Pérez-García, F., Brito, L. F., Irla, M., Jorge, J. M., & Sgobba, E. (2022). Promising and sustainable microbial feedstocks for biotechnological processes. Frontiers in Microbiology, 13, 973723. DOI: https://doi.org/10.3389/fmicb.2022.973723
Rukwishuro, T., Musengi, A., Ndemera, M., Mugiyo, H., & Gonde, L. (2025). A systematic review on indigenous starter cultures that have been applied in the traditional fermentation of dairy products in sub-Saharan Africa. Food Research International, 218, 116743. DOI: https://doi.org/10.1016/j.foodres.2025.116743
Simbine-Ribisse, E. O., Bila, N. M., Manhiça, A. J., Macuamule, C. J., & Bragotto, A. P. A. (2024). Antibiotic residues in dairy products in Africa: A systematic review. Food Chemistry Advances, 5, 100822. DOI: https://doi.org/10.1016/j.focha.2024.100822
Stabnikov, V., Kovshar, I., & Stabnikova, O. (2025). Recent advances in the study of the properties and applications of lactic acid bacteria. World Journal of Microbiology and Biotechnology, 41(8), 287. DOI: https://doi.org/10.1007/s11274-025-04499-0
Uzeh, R. E., & Imafidon, S. (2022). The occurrence of antibiotic-resistant enteric bacteria in selected Nigerian traditional dairy products. African Health Sciences, 22(4), 619. DOI: https://doi.org/10.4314/ahs.v22i4.67
Uzoaga, G. O., Umeokonkwo, C. D., Kia, G. S., & Okolocha, E. C. (2020). Bacteriological quality of Nono, a milk product sold at retail outlets in Federal Capital Territory, Nigeria. Journal of Interventional Epidemiology and Public Health, 3(1), 5-5. DOI: https://doi.org/10.37432/JIEPH.2020.3.2.25
Vantsawa, P.A., Maryah, U.T., and Timothy, B. (2017). Isolation and Identification of Lactic Acid Bacteria with Probiotic Potential from Fermented Cow Milk (Nono) in Unguwar Rimi, Kaduna State, Nigeria. American Journal of Molecular Biology, 7, 99-106. DOI: https://doi.org/10.4236/ajmb.2017.72008
Vénica, C. I., Wolf, I. V., Beret, M. V., Bergamini, C. V., Burns, P., Binetti, A., & Perotti, M. C. (2023). Influence of commercial starter culture on fermentation dynamics and quality characteristics of yogurts obtained with different formulations. Journal of the Science of Food and Agriculture, 103(2), 569-575. DOI: https://doi.org/10.1002/jsfa.12168
World Health Organization. (2026). Geographical map of Zaria and Sabon Gari Local Government Areas (LGAs), Kaduna State, Nigeria, highlighting major roads, markets, and community landmarks relevant to informal dairy marketing [Map]. World Health Organization. Available at: https://gis-who.hub.arcgis.com/pages/map-request-page
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Copyright (c) 2026 Muhammad Muhsin Fathuddin, Saleh Alhaji Ado, Muhammad Bashir Tijjani, Haruna Makonjuola Kazeem (Author)

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